Potency of hyaluronic acid from eggshell-membrane for open gingival embrasure reconstruction following orthodontic tooth movement (a histomorphological study)

The aim of this research was to assess the effectiveness of eggshell-membrane (ESM)-containing hyaluronic acid (HA) in the treatment of open gingival embrasure (OGE) following orthodontic tooth movement (OTM). This study is an quasi experimental research. A total of 24 were equally divided into two...

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Bibliographic Details
Published in:Dental research journal 2022, Vol.19 (1), p.107-107
Main Authors: Suparwitri, Sri, Alhasyimi, Ananto Ali
Format: Article
Language:English
Subjects:
Ethylenediaminetetraacetic acid
Hyaluronic acid
open gingival embrasure
Original
orthodontic tooth movement
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Summary:The aim of this research was to assess the effectiveness of eggshell-membrane (ESM)-containing hyaluronic acid (HA) in the treatment of open gingival embrasure (OGE) following orthodontic tooth movement (OTM). This study is an quasi experimental research. A total of 24 were equally divided into two groups, treatment (10% HA injection) and control (phosphate-buffered saline [PBS]). A separator was inserted between mandibular incisors to induce an OGE. A volume of 20 μl of either PBS ( = 12) or ESM extract ( = 12) was locally injected within the interdental papilla. Decapitation of animals was made on day 1, 4, and 7 postinjection. The staining was done using hemotoxylin and eosin to observe angiogenesis and Mallory to observe the collagen density. Fourier-transform infrared spectroscopy (FTIR) and thin-layer chromatography (TLC) analysis were performed to detect the amount of HA available in ESM. The results were then compared with independent -tests and the Mann-Whitney test. The level of statistical significance was set at 0.05. The FTIR and TLC analysis showed that HA was successfully identified in the ESM samples. Local injection of 10% HA induced an increase of angiogenesis compared to the control group on day 1 and 4 postinjection ( < 0.05). Significant differences ( < 0.05) were also noted in the collagen density and the growth of interdental papilla on day 4 and 7 postinjection. ESM has the potential effect of regenerating the interdental papilla construction after OTM by increasing the collagen fiber density and inducing angiogenesis.
ISSN:1735-3327
2008-0255
DOI:10.4103/1735-3327.363566