Development of an algorithm for biomedical image analysis of the spatial organization of collagen in breast cancer tissue of patients with different clinical status

Collagen, the main component of the tumor microenvironment, plays a key role in the development of breast cancer (BCa); however, the specific changes in its spatial organization during tumor progression have not been definitively elucidated. The existing and available methods for assessing the morph...

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Bibliographic Details
Published in:FEBS open bio 2024-04, Vol.14 (4), p.675-686
Main Authors: Lukianova, Nataliia, Mushii, Oleksandr, Zadvornyi, Taras, Chekhun, Vasyl
Format: Article
Language:English
Subjects:
Algorithms
Breast Cancer
Cancer Diagnosis
Collagen
Fibers
Image processing
microphoto analysis
Microscopy
Oncology
Software packages
spatial organization
Stains & staining
Stroma
Tumor microenvironment
tumor progression
Tumors
tumor‐stroma ratio
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Summary:Collagen, the main component of the tumor microenvironment, plays a key role in the development of breast cancer (BCa); however, the specific changes in its spatial organization during tumor progression have not been definitively elucidated. The existing and available methods for assessing the morphometric parameters of the stroma's fibrous component are insufficient for a detailed description of the state of collagen fibers and for assessing its changes to evaluate the aggressiveness of the BCa course. The aim of the work was to develop an algorithm for microphoto analysis to assess the spatial organization of collagen in BCa tissue of patients with different clinical statuses. The study was conducted on 60 tissue samples of stage I‐II BCa. The processed images were analyzed using the software packages CurveAlign v4.0 and imagej. We established that the increase in BCa stage and the decrease in tumor differentiation grade are associated with decreased length, width, and straightness of collagen fibers, as well as their increased density. The formation of an aggressive basal molecular BCa subtype was accompanied by an increase in tumor‐stroma ratio. The obtained results indicate the possibility of practical application of the developed algorithm for evaluating the spatial organization of collagen in BCa tissue to predict the aggressiveness of the disease course. In order to assess the condition of the tumor collagen matrix, we developed an algorithm for microphoto analysis, which uses Malory's trichrome staining, and also involves specialized software packages (curve align and imagej) for morphometric studies. After completing the analysis, we obtained morphometric indicators of the mammary gland malignant neoplasms stromal fibrous components.
ISSN:2211-5463
2211-5463
DOI:10.1002/2211-5463.13773