The BCO2 Genotype and the Expression of BCO1 , BCO2 , LRAT, and TTPA Genes in the Adipose Tissue and Brain of Rabbits Fed a Diet with Marigold Flower Extract

• Published in: International journal of molecular sciences 2023-01, Vol.24 (3), p.2304
• Main Authors: Strychalski, Janusz, Gugołek, Andrzej, Kaczorek-Łukowska, Edyta, Antoszkiewicz, Zofia, Matusevičius, Paulius
• Format: Article
• Language: English
• Subjects: Adipose tissue; Adipose Tissue - metabolism; alpha-Tocopherol - metabolism; Animals; BCO2 gene; beta-Carotene 15,15'-Monooxygenase - genetics; Biosynthesis; Body fat; Brain; Brain - metabolism; Carotene; Carotenoids; Diet; Dioxygenases - genetics; Enzymes; Flowers; Flowers & plants; Gene expression; Genes; Genotype; Genotype & phenotype; Genotypes; Lutein; Male; marigold; Marigolds; Metabolism; Proteins; Rabbits; Subgroups; Tissue Plasminogen Activator - metabolism; Tocopherol; Vitamin A; Vitamin E; Zeaxanthin; β-Carotene
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24032304

This study was undertaken to evaluate the effect of the genotype and dietary supplementation with marigold flower extract on the expression of , , and genes in the adipose tissue and brain of rabbits. The concentrations of lutein, zeaxanthin, β-carotene, retinol, and α-tocopherol were determined in samples collected from rabbits. Sixty young male Termond White rabbits were allocated to three groups based on their genotype at codon 248 of the gene (ins/ins, ins/del, and del/del). Each group comprised two subgroups; one subgroup was administered a standard diet, whereas the diet offered to the other subgroup was supplemented with 6 g/kg of marigold flower extract. The study demonstrated that the genotype may influence the expression levels of the , and genes in adipose tissue, and and genes in the brain. Moreover, an increase in the amount of lutein in the diet of BCO2 del/del rabbits may increase the expression of , and genes in adipose tissue, and the expression of the gene in the brain. Another finding of the study is that the content of carotenoids and α-tocopherol increases in both the adipose tissue and brain of del/del rabbits.