α-Solanine Causes Cellular Dysfunction of Human Trophoblast Cells via Apoptosis and Autophagy

The trophoblast, an embryonic tissue, exerts a crucial role in the processes of implantation and placentation. Toxins in food can cause malfunction of trophoblasts, resulting in apoptosis, oxidative stress, and abnormal angiogenesis. α-solanine, a steroidal glycoalkaloid, has antitumor properties on...

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Bibliographic Details
Published in:Toxins 2021-01, Vol.13 (1), p.67
Main Authors: Chen, Zhilong, Li, Chen, Yuan, Anwen, Gu, Ting, Zhang, Feng, Fan, Xiujun, Wu, Xiaosong, Xiong, Xingyao, Yang, Qing
Format: Article
Language:English
Subjects:
Abortion
Angiogenesis
Anticancer properties
Apoptosis
Autophagy
Biomarkers
Cancer
Cell activation
Cell cycle
Cell growth
Cell migration
Cell proliferation
Cell viability
Cytotoxicity
Embryos
Fetuses
Food cans
Gene expression
Glycoalkaloids
Microtubule-associated protein 1
Oxidative stress
Phagocytosis
Pregnancy
Reproductive system
toxicity
Toxins
trophoblast cell
Trophoblasts
α-solanine
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Summary:The trophoblast, an embryonic tissue, exerts a crucial role in the processes of implantation and placentation. Toxins in food can cause malfunction of trophoblasts, resulting in apoptosis, oxidative stress, and abnormal angiogenesis. α-solanine, a steroidal glycoalkaloid, has antitumor properties on several cancer cells. However, its effect on human trophoblasts has not been elucidated. In this study, human extravillous trophoblast HTR-8/SVneo cells were exposed to α-solanine. Cellular functions including proliferation, migration, invasion, tube formation, and apoptosis were assessed. To monitor autophagic flux, trophoblasts were transfected with a mCherry-GFP-LC3B vector using lentiviral transduction, and expression of autophagy-related biomarkers including Beclin 1, Atgl3, and microtubule-associated protein 1 light chain-3 (MAP1-LC3) were detected. The results show that application of 20 μM α-solanine or above inhibited the cell viability, migration, invasion, and tube formation of the human trophoblast. Cell cycle was arrested at S and G2/M phases in response to 30 μM α-solanine. α-solanine induced apoptosis of HTR-8/SVneo cells and triggered autophagy by increasing the autophagic gene expression and stimulating the formation of autophagosome and autophagic flux. In conclusion, α-solanine can impair the functions of human trophoblast cells via activation of cell apoptosis and autophagy.
ISSN:2072-6651
2072-6651
DOI:10.3390/TOXINS13010067