Antiviral Activity of Angelica Tenuissima Nakai against Influenza A Virus

The influenza A virus poses a serious threat to human health and is an important global public health issue. The drugs currently used for treatment are becoming increasingly ineffective against influenza A viruses and require the development of new antiviral drugs. Angelica tenuissima Nakai (ATN), a...

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Bibliographic Details
Published in:Pathogens (Basel) 2024-09, Vol.13 (9), p.761
Main Authors: Park, Won-Jong, Bae, Gi-Sang, Han, Youn-Ho
Format: Article
Language:English
Subjects:
adsorption
Angelica
Angelica tenuissima Nakai
antiviral
Antiviral activity
Antiviral agents
Antiviral drugs
antiviral properties
cold
Colorimetry
Cytotoxicity
dogs
Drug development
Drug dosages
Drug resistance
Drugs
family
Gene set enrichment analysis
Genes
headache
Health aspects
Herbal medicine
herbal medicines
human health
Infections
Infectious diseases
inflammation
Influenza
Influenza A
Influenza A virus
kidneys
Kinases
Medicine, Botanic
Medicine, Herbal
microscopy
Mortality
Pandemics
Performance evaluation
Pharmacology
Proteins
Public health
RNA
RNA viruses
Statistical analysis
Sulforhodamine
Survival
survival rate
tetrazolium
therapeutics
Vaccines
Viral infections
Viruses
Citations: Items that this one cites
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Description
Summary:The influenza A virus poses a serious threat to human health and is an important global public health issue. The drugs currently used for treatment are becoming increasingly ineffective against influenza A viruses and require the development of new antiviral drugs. Angelica tenuissima Nakai (ATN), a traditional herbal medicine belonging to the Umbelliferae family, exhibits a broad range of pharmacological activities, including inflammation, headache, and cold symptoms. In the present study, based on target protein identification, functional enrichment analysis, and gene set comparisons, we first suggested that ATN has potential therapeutic effects against influenza A virus infection. Next, methylthiazol tetrazolium (MTT) and sulforhodamine B colorimetric (SRB) assay results revealed that ATN exhibited low cytotoxicity in Madin-Darby canine kidney (MDCK) cells. The antiviral properties of ATN were observed against H1N1 and H3N2 virus strains. Microscopy confirmed the increased survival rate of the host cells. Further time-of-addition experiments revealed that the addition of ATN before virus adsorption showed similar results to the whole period of treatment. The pre- and co-treated groups showed lower levels of viral RNA (M1 protein). The results of this study suggest that ATN exhibits antiviral properties against the influenza A virus. These therapeutic properties of ATN can serve as a theoretical basis for further research on the applicability of ATN in the development of antiviral agents.
ISSN:2076-0817
2076-0817
DOI:10.3390/pathogens13090761