Protocol for behavioral and neural recording in macaques during a novelty detection task

• Published in: STAR protocols 2024-09, Vol.5 (3), p.103252, Article 103252
• Main Authors: Du, Xinyu, Song, Peirun, Gong, Yumei, Zhai, Yuying, Xu, Haoxuan, Ye, Hangting, Bao, Xuehui, Huang, Qianyue, Tu, Zhiyi, Chen, Pei, Zhao, Xuan, Pérez-González, David, Malmierca, Manuel S., Yu, Xiongjie
• Format: Article
• Language: English
• Subjects: Animals; Auditory Cortex - diagnostic imaging; Auditory Cortex - physiology; Behavior; Behavior, Animal - physiology; Cognitive Neuroscience; Electrodes, Implanted; Exploratory Behavior - physiology; Macaca; Macaca mulatta; Magnetic Resonance Imaging - methods; Male; Neurons - physiology; Neuroscience; Protocol
• ISSN: 2666-1667; 2666-1667
• DOI: 10.1016/j.xpro.2024.103252

The neural mechanisms of novelty detection, especially in relation to behavior, are currently poorly understood. Here, we present a protocol for recording neuronal activity in macaque auditory cortex during novelty detection tasks. We describe steps for behavioral training, surgical headpost implantation, MRI-based electrode targeting, and electrophysiological recording. These steps allow direct assessment of the correlation between novelty detection behavior and neuronal activity. For complete details on the use and execution of this protocol, please refer to Gong et al.1 [Display omitted] •A protocol for recording neuronal activity in macaques during novelty detection tasks•Precise electrode implantation using MRI targeting•Detailed steps for headpost installation and behavioral task design•Assessing the link between novelty detection behavior and neuronal activity Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. The neural mechanisms of novelty detection, especially in relation to behavior, are currently poorly understood. Here, we present a protocol for recording neuronal activity in macaque auditory cortex during novelty detection tasks. We describe steps for behavioral training, surgical headpost implantation, MRI-based electrode targeting, and electrophysiological recording. These steps allow direct assessment of the correlation between novelty detection behavior and neuronal activity.