Potential use of quantum dots in flow cytometry

QDs may offer significant advantages in environmental and bead-based applications where the target cells need to be discriminated above background fluorescence. We have examined the possible applications of QDs for flow cytometric measurements (FCM) by studying their excitation - emission spectra an...

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Bibliographic Details
Published in:International journal of molecular sciences 2008-12, Vol.9 (12), p.2622-2638
Main Authors: Ibáñez-Peral, Raquel, Bergquist, Peter L, Walter, Malcolm R, Gibbs, Moreland, Goldys, Ewa M, Ferrari, Belinda
Format: Article
Language:English
Subjects:
Antibodies
autofluorescence
Cadmium selenide
detection limit
Dyes
Electrons
Flow cytometry
fluorescent detection
Microorganisms
Optical properties
Quantum dots
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Summary:QDs may offer significant advantages in environmental and bead-based applications where the target cells need to be discriminated above background fluorescence. We have examined the possible applications of QDs for flow cytometric measurements (FCM) by studying their excitation - emission spectra and their binding to paramagnetic beads. We labelled beads with either QDs or a commonly-used fluorochrome (FITC) and studied their fluorescence intensity by FCM. Flow cytometric comparisons indicated that the minimum fluorophore concentration required for detection of QDs above autofluorescent background was 100-fold less than for FITC.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms9122622