Loading…

Agronomical and molecular characterization of banana germplasm Caracterização agronômica e molecular de germoplasma de bananeira

The objective of the present work was to characterize banana accessions from the Germplasm Bank at Embrapa Mandioca e Fruticultura Tropical (Brazil), using agronomical, physical and physicochemical characteristics of fruit and simple sequence repeats (SSR) markers. Twenty-six accessions were analyze...

Full description

Saved in:
Bibliographic Details
Published in:Pesquisa agropecuaria brasileira 2010-02, Vol.45 (2), p.146-154
Main Authors: Lorenna Alves Mattos, Edson Perito Amorim, Vanusia Batista de Oliveira Amorim, Kelly de Oliveira Cohen, Carlos Alberto da Silva Ledo, Sebastião de Oliveira e Silva
Format: Article
Language:English
Subjects:
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The objective of the present work was to characterize banana accessions from the Germplasm Bank at Embrapa Mandioca e Fruticultura Tropical (Brazil), using agronomical, physical and physicochemical characteristics of fruit and simple sequence repeats (SSR) markers. Twenty-six accessions were analyzed, in which high genetic variability was found, especially for the agronomical characters number of fruit and weight of bunch. Accessions with high contents of carotenoids (diploid 'Jaran'), polyphenols (triploid 'Caipira' and tetraploid 'Teparod') and vitamin C (diploid 'Tuugia' and an unknown triploid AAA) in the fruit were identified. Thirteen microsatellite primers revealed an average of 7.23 alleles, which showed high variability. A dendrogram was prepared using the Gower algorithm for the distance matrices obtained from the agronomical, physical and physicolchemical analysis of fruit and SSR markers. Adopting the average genetic divergence as the cut-off point, three clusters were found: G1, formed by the diploids 'Jaran', 028003-01 and M-48; G2, by the diploids 'Malbut' and 'Ido 110'; and G3, by 21 tri-and tetraploid accessions, including one diploid, 'Tuugia'. The triploids with the B genome 'Thap Maeo', 'Walha', 'Pacha Nadan' and 'Champa Madras' were grouped in G2. Results from this work can be used for breeding hybrids with good agronomical traits and fruit quality.O objetivo deste trabalho foi caracterizar acessos de bananeira do Banco de Germoplasma da Embrapa Mandioca e Fruticultura Tropical, por meio de características agronômicas, físicas e físico-químicas dos frutos e por marcadores "Simple sequence repeats" (SSR). Foram analisados 26 acessos, nos quais observou-se ampla variabilidade genética, em especial para número de frutos e peso de cacho. Foram identificados acessos com altos teores de carotenoides (diploide 'Jaran'), polifenóis (tetraploide 'Teparod') e vitamina C (diploide 'Tuugia' e um triploide AAA desconhecido). Os 13 iniciadores microssatélites testados apresentaram uma média de 7,23 alelos, que apresentaram alta variabilidade. Com uso do algoritmo de Gower, foi elaborado um dendrograma com as matrizes de distância obtidas por meio das análises agronômicas, físicas e físico-químicas dos frutos e análises moleculares. Com a divergência genética média usada como ponto de corte, foram identificados três agrupamentos: G1, formado pelos diploides 'Jaran', 028003-01 e M-48; G2, pelos diploides 'Malbut' e 'Ido 110'; e G3, pelos 21 acessos tr
ISSN:0100-204X
1678-3921
DOI:10.1590/S0100-204X2010000200005