Comparative analysis of the main haematological indexes and RNA detection for the diagnosis of SARS-CoV-2 infection

• Published in: BMC infectious diseases 2020-10, Vol.20 (1), p.779-779, Article 779
• Main Authors: Xiang, Jialin, Chen, Zuyi, Zhou, Jie, Tian, Di, Ran, Xiusheng, Zhang, Zhimin, Shi, Shi, Xiao, Daimin, Zhou, Yuanzhong
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Antibodies; Antibodies, Viral - blood; Antibody; Automation; Betacoronavirus - genetics; Betacoronavirus - immunology; Blood; Blood cells; C-reactive protein; Clinical Laboratory Techniques; Cohort Studies; Comparative analysis; Coronaviridae; Coronavirus Infections - diagnosis; Coronavirus Infections - virology; Coronaviruses; Correlation analysis; COVID-19; COVID-19 Testing; Diagnostic systems; Enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Enzymes; Female; Genes; Gold immunochromatography assay; Health aspects; Hematology; Humans; Immunoglobulin G; Immunoglobulin G - blood; Immunoglobulin M; Immunoglobulin M - blood; Indicators; Infection; Infections; Laboratories; Leukocytes; Lymphocytes; Male; Medical research; Medical schools; Middle Aged; Neutrophils; Nucleic acids; Pandemics; Patients; Pneumonia; Pneumonia, Viral - diagnosis; Pneumonia, Viral - virology; Public health; Real-time PCR; Real-Time Polymerase Chain Reaction - methods; Regression analysis; Respiratory diseases; Ribonucleic acid; RNA; RNA, Viral - genetics; SARS-CoV-2; Sensitivity; Sensitivity and Specificity; Severe acute respiratory syndrome; Severe acute respiratory syndrome coronavirus 2; Signs and symptoms; Statistical analysis; Statistical methods; Viral diseases; Viruses
• ISSN: 1471-2334; 1471-2334
• DOI: 10.1186/s12879-020-05489-3

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has become a public health emergency of international concern. SARS-CoV-2 RNA detection is the diagnostic criterion for coronavirus disease 2019 (COVID-19). Nevertheless, RNA detection has many limitations, such as being time-consuming and cost-prohibitive, and it must be performed in specialized laboratories. Virus antibody detection is a routine method for screening for multiple viruses, but data about SARS-CoV-2 antibody detection are limited. Throat swabs and blood were collected from 67 suspected SARS-CoV-2 infection patients at the Affiliated Hospital of Zunyi Medical University and Zunyi Fourth People's Hospital isolated observation departments. Throat swab samples were subjected to SARS-CoV-2 RNA detection by real-time PCR. Blood was used subjected to SARS-CoV-2 IgG/IgM detection by an enzyme-linked immunosorbent assay (ELISA) and gold immunochromatography assay (GICA). Blood underwent C-reactive protein detection by immunoturbidimetry, and white blood cells, neutrophil percentages and lymphocyte percentages were counted and calculated, respectively. Clinical symptoms, age and lifestyle habits (smoking and drinking) in all patients were recorded. Data were analysed using SPSS version 19. The results were confirmed by T and χ tests; correlations with detection results were analysed by kappa coefficients. Odds ratio (OR) and corrected OR values were analysed by logistic regression. P