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An updated view into the cell cycle kinetics of human T lymphocytes and the impact of irradiation

Even though a detailed understanding of the proliferative characteristics of T lymphocytes is imperative in many research fields, prior studies have never reached a consensus on these characteristics, and on the corresponding cell cycle kinetics specifically. In this study, the general proliferative...

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Published in:Scientific reports 2022-05, Vol.12 (1), p.7687-7687, Article 7687
Main Authors: Duthoo, Evi, Vral, Anne, Baeyens, Ans
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description Even though a detailed understanding of the proliferative characteristics of T lymphocytes is imperative in many research fields, prior studies have never reached a consensus on these characteristics, and on the corresponding cell cycle kinetics specifically. In this study, the general proliferative response of human T lymphocytes to phytohaemagglutinin (PHA) stimulation was characterized using a carboxyfluorescein succinimidyl ester-based flow cytometric assay. We were able to determine when PHA-stimulated T lymphocytes complete their first division, the proportion of cells that initiate proliferation, the subsequent division rate of the cells, and the impact of irradiation on these proliferative properties. Next, we accurately visualized the cell cycle progression of dividing T lymphocytes cultured in whole blood using an adapted 5-ethynyl-2’-deoxyuridine pulse-chase method. Furthermore, through multiple downstream analysis methods, we were able to make an estimation of the corresponding cell cycle kinetics. We also visualized the impact of X-rays on the progression of the cells through the cell cycle. Our results showed dose-dependent G2 arrest after exposure to irradiation, and a corresponding delay in G1 phase-entry of the cells. In conclusion, utilizing various flow cytometric assays, we provided valuable information on T lymphocyte proliferation characteristics starting from first division to fully dividing cells.
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subjects 631/1647/1407/1492
631/80/641/2187
Cell cycle
Cell proliferation
Flow cytometry
G1 phase
Humanities and Social Sciences
Irradiation
Kinetics
Lymphocytes
Lymphocytes T
multidisciplinary
Science
Science (multidisciplinary)
title An updated view into the cell cycle kinetics of human T lymphocytes and the impact of irradiation
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