Recognition Pattern of the Fasciola hepatica Excretome/Secretome during the Course of an Experimental Infection in Sheep by 2D Immunoproteomics

• Published in: Pathogens (Basel) 2021-06, Vol.10 (6), p.725
• Main Authors: Becerro-Recio, David, González-Miguel, Javier, Ucero, Alberto, Sotillo, Javier, Martínez-Moreno, Álvaro, Pérez-Arévalo, José, Cwiklinski, Krystyna, Dalton, John P., Siles-Lucas, Mar
• Format: Article
• Language: English
• Subjects: Antigens; Brief Report; Calcium-binding protein; Calmodulin; Cathepsin L; Diagnostic systems; Electrophoresis; excretory/secretory antigens; Experimental infection; Fasciola hepatica; Ferritin; Glutathione; Glutathione transferase; Immune response; Immune system; Immunogenicity; Immunomodulation; immunoproteomics; Infections; Isoforms; Liver; Mass spectrometry; Mass spectroscopy; Molecular interactions; Parasites; Pattern recognition; Peroxiredoxin; Proteins; Secretions; Secretome; Sheep; Superoxide dismutase; Therapeutic targets
• ISSN: 2076-0817; 2076-0817
• DOI: 10.3390/pathogens10060725

Excretory/secretory products released by helminth parasites have been widely studied for their diagnostic utility, immunomodulatory properties, as well as for their use as vaccines. Due to their location at the host/parasite interface, the characterization of parasite secretions is important to unravel the molecular interactions governing the relationships between helminth parasites and their hosts. In this study, the excretory/secretory products from adult worms of the trematode Fasciola hepatica (FhES) were employed in a combination of two-dimensional electrophoresis, immunoblot and mass spectrometry, to analyze the immune response elicited in sheep during the course of an experimental infection. Ten different immunogenic proteins from FhES recognized by serum samples from infected sheep at 4, 8, and/or 12 weeks post-infection were identified. Among these, different isoforms of cathepsin L and B, peroxiredoxin, calmodulin, or glutathione S-transferase were recognized from the beginning to the end of the experimental infection, suggesting their potential role as immunomodulatory antigens. Furthermore, four FhES proteins (C2H2-type domain-containing protein, ferritin, superoxide dismutase, and globin-3) were identified for the first time as non-immunogenic proteins. These results may help to further understand host/parasite relationships in fasciolosis, and to identify potential diagnostic molecules and drug target candidates of F. hepatica.