Analysis of Phenolic Composition of Byproducts (Seeds and Peels) of Avocado ( Persea americana Mill.) Cultivated in Colombia

The byproducts (seeds and peels) of an avocado cultivated in the south of Colombia were extracted with aqueous acetone and their antioxidant properties were measured with ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and DPPH (2,2-diphenyl-1-picrylhydrazyl) assays, and total pol...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2019-09, Vol.24 (17), p.3209
Main Authors: Rosero, Johanna C, Cruz, Silvia, Osorio, Coralia, Hurtado, Nelson
Format: Article
Language:English
Subjects:
Acetone
Antioxidants
bio-guided fractionation
Byproducts
Catechin
Catechin - chemistry
Colombia
Coumaric Acids - chemistry
Cultivars
DPPH
Fractionation
Freeze drying
Inflammation
Lipid metabolism
Lipids
Maceration
Organic chemistry
Oxidants
Oxidizing agents
Persea - chemistry
Phenolic compounds
Phenols
Phenols - chemistry
Plant extracts
Platelet aggregation
Polyphenols
Polyphenols - chemistry
Proanthocyanidins - chemistry
Seeds
Seeds - chemistry
Size exclusion chromatography
Solid phases
Solvent extraction
Solvents
Tandem Mass Spectrometry - methods
Tonnage
Trolox equivalent antioxidant capacity (TEAC)
UPLC-ESI-MS/MS
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Summary:The byproducts (seeds and peels) of an avocado cultivated in the south of Colombia were extracted with aqueous acetone and their antioxidant properties were measured with ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and DPPH (2,2-diphenyl-1-picrylhydrazyl) assays, and total polyphenol content was determined by Folin-Ciocalteu method. A bioguided fractionation was performed, first by SPE (solid phase extraction) on Amberlite XAD-7, and then by size exclusion chromatography on Sephadex LH-20. The polyphenolic-rich extracts and their fractions were analyzed by ultra-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS/MS), finding the presence of organic acids, hydroxycinnamic acids, catechins, free and glycosylated flavonoids, and dimeric and trimeric procyanidins. Catechin, epicatechin, six quercetin derivatives, four dimeric procyanidins (three type B and one type A), and three trimeric procyanidins (two type B and one type A) were detected in the most active fractions of avocado peel and seeds. The most antioxidant fractions contain the higher molecular weight phenolic compounds (condensed tannins).
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules24173209