Lysine-specific demethylase 1 inhibition enhances autophagy and attenuates early-stage post-spinal cord injury apoptosis

Neuron death in spinal cords is caused primarily by apoptosis after spinal cord injury (SCI). Autophagy can act as a cellular response to maintain neuron homeostasis that can reduce apoptosis. Although more studies have shown that an epigenetic enzyme called Lysine-specific demethylase 1 (LSD1) can...

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Bibliographic Details
Published in:Cell death discovery 2021-04, Vol.7 (1), p.69-69, Article 69
Main Authors: Gu, Yang, Chen, Dehui, Zhou, Linquan, Zhao, Xin, Lin, Jiemin, Lin, Bin, Lin, Taotao, Chen, Zhi, Chen, Zhaohong, Wang, Zhenyu, Liu, Wenge
Format: Article
Language:English
Subjects:
631/378/1934
631/378/87
692/53/2422
692/699/578
Animal models
Apoptosis
Autophagy
Biochemistry
Biomedical and Life Sciences
Cancer research
Cell Biology
Cell Cycle Analysis
Cell death
Epigenetics
Homeostasis
Life Sciences
Lysine
Medical research
Neuroprotection
Phagocytosis
Spinal cord injuries
Stem Cells
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Summary:Neuron death in spinal cords is caused primarily by apoptosis after spinal cord injury (SCI). Autophagy can act as a cellular response to maintain neuron homeostasis that can reduce apoptosis. Although more studies have shown that an epigenetic enzyme called Lysine-specific demethylase 1 (LSD1) can negatively regulate autophagy during cancer research, existing research does not focus on impacts related to LSD1 in nerve injury diseases. This study was designed to determine whether inhibiting LSD1 could enhance autophagy against apoptosis and provide effective neuroprotection in vitro and vivo after SCI. The results showed that LSD1 inhibition treatment significantly reduced spinal cord damage in SCI rat models and was characterized by upregulated autophagy and downregulated apoptosis. Further research demonstrated that using both pharmacological inhibition and gene knockdown could enhance autophagy and reduce apoptosis for in vitro simulation of SCI-caused damage models. Additionally, 3-methyladenine (3-MA) could partially eliminate the effect of autophagy enhancement and apoptosis suppression. These findings demonstrated that LSD1 inhibition could protect against SCI by activating autophagy and hindering apoptosis, suggesting a potential candidate for SCI therapy.
ISSN:2058-7716
2058-7716
DOI:10.1038/s41420-021-00455-7