Gene Expression Correlation Analysis Reveals MYC-NAC Regulatory Network in Cotton Pigment Gland Development

Plant NAC (NAM, ATAF1/2, and CUC2) family is involved in various development processes including Programmed Cell Death (PCD) associated development. However, the relationship between NAC family and PCD-associated cotton pigment gland development is largely unknown. In this study, we identified 150,...

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Published in:International journal of molecular sciences 2021-05, Vol.22 (9), p.5007
Main Authors: Cheng, Hailiang, Feng, Xiaoxu, Zuo, Dongyun, Zhang, Youping, Wang, Qiaolian, Lv, Limin, Wu, Chaofeng, Li, Shuyan, Dai, Yuanli, Qu, Da, He, Man, Liu, Shang, Song, Guoli
Format: Article
Language:English
Subjects:
Amino acids
Apoptosis
Biosynthesis
Cell death
Chromosomes
Correlation analysis
Cotton
cotton pigment gland
Deoxyribonucleic acid
DNA
Gene expression
Genes
Genomes
Genotype & phenotype
GhMYC2-like
Glands
Myc protein
NAC transcription factors
Phenotypes
Phylogenetics
Phylogeny
Plant resistance
Proteins
regulatory network
Senescence
Transcription factors
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Summary:Plant NAC (NAM, ATAF1/2, and CUC2) family is involved in various development processes including Programmed Cell Death (PCD) associated development. However, the relationship between NAC family and PCD-associated cotton pigment gland development is largely unknown. In this study, we identified 150, 153 and 299 NAC genes in newly updated genome sequences of G. arboreum, G. raimondii and G. hirsutum, respectively. All NAC genes were divided into 8 groups by the phylogenetic analysis and most of them were conserved during cotton evolution. Using the vital regulator of gland formation GhMYC2-like as bait, expression correlation analysis screened out 6 NAC genes which were low-expressed in glandless cotton and high-expressed in glanded cotton. These 6 NAC genes acted downstream of GhMYC2-like and were induced by MeJA. Silencing CGF1(Cotton Gland Formation1), another MYC-coding gene, caused almost glandless phenotype and down-regulated expression of GhMYC2-like and the 6 NAC genes, indicating a MYC-NAC regulatory network in gland development. In addition, predicted regulatory mechanism showed that the 6 NAC genes were possibly regulated by light, various phytohormones and transcription factors as well as miRNAs. The interaction network and DNA binding sites of the 6 NAC transcription factors were also predicted. These results laid the foundation for further study of gland-related genes and gland development regulatory network.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22095007