Evaluation of the Diuretic Activity of Aqueous and 80% Methanol Extracts of Croton macrostachyus (Euphorbiaceae) Leaves in Saline-Loaded Rats

(Euphorbiaceae) extract is a folk medicine traditionally used for treating a number of disorders, including edematous conditions. The present study aimed to evaluate the diuretic effects of aqueous and 80% methanol leaf extracts of in saline-loaded rats. Rats of either sex were randomly assigned int...

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Bibliographic Details
Published in:Journal of experimental pharmacology 2021, Vol.13, p.213-221
Main Authors: Tufer, Sara, Engidawork, Ephrem, Ayele, Akeberegn Gorems, Bashea, Chala
Format: Article
Language:English
Subjects:
Animals
croton macrostachuys del
diuretic activity
Diuretics
Drug dosages
Electrolysis
electrolyte
Electrolytes
Furosemide
Heart failure
Herbal medicine
Laboratories
leaf
Metabolism
Methanol
Original Research
Pharmacy
Potassium
rats
Urine
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Summary:(Euphorbiaceae) extract is a folk medicine traditionally used for treating a number of disorders, including edematous conditions. The present study aimed to evaluate the diuretic effects of aqueous and 80% methanol leaf extracts of in saline-loaded rats. Rats of either sex were randomly assigned into eight groups of eight animals per group. The animals were treated with vehicle (distilled water), standard (furosemide 10 mg/kg), and three doses (100, 200, and 400 mg/kg) of aqueous and 80% methanol leaf extracts after loading of normal saline (15 mL/kg). Then, urine volume, electrolyte concentration, and pH were measured as parameters of evaluation. Concentrations of urinary Na K , and Cl were determined and Na :K and Cl :Na + K ratios calculated to reveal possible mechanisms. The aqueous extract at 200 mg/kg had produced significant diuresis by hour 3, while the same dose of 80% methanol extract had produced substantial diuresis by the end of hour 4. Both extracts at 400 mg/kg produced significant diuresis from hour 2 to hour 5. In terms of effect on electrolysis, 400 mg/kg aqueous extract produced significant natriuresis, and a kaliuresis effect was observed for both extracts at higher doses and 200 mg/kg aqueous extract. The findings collectively indicated that both aqueous and 80% methanol extract showed significant diuretic activity, thereby justifying the plant's traditional use as a diuretic agent.
ISSN:1179-1454
1179-1454
DOI:10.2147/JEP.S294062