Oral Clostridium butyricum on mice endometritis through uterine microbiome and metabolic alternations

• Published in: Frontiers in microbiology 2024-02, Vol.15, p.1351899-1351899
• Main Authors: Hagihara, Mao, Ariyoshi, Tadashi, Eguchi, Shuhei, Oka, Kentaro, Takahashi, Motomichi, Kato, Hideo, Shibata, Yuichi, Umemura, Takumi, Mori, Takeshi, Miyazaki, Narimi, Hirai, Jun, Asai, Nobuhiro, Mori, Nobuaki, Mikamo, Hiroshige
• Format: Article
• Language: English
• Subjects: Clostridium butyricum; endometritis; Lactobacillus species; metabolome; Microbiology; microbiome; resolvin D5
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2024.1351899

Endometritis occurs frequently in humans and animals, which can negatively affect fertility and cause preterm parturition syndrome. Orally administered , a butyrate-producing gram-positive anaerobe, exhibits anti-inflammatory effects. However, the precise mechanism by which attenuates endometritis remains unclear. This study evaluated the anti-inflammatory effects of orally administered on uterine tissues. In addition, we conducted uterine microbiome and lipid metabolome analyses to determine the underlying mechanisms. Female Balb/c mice were divided into the following four groups (  = 5-20): (1) mock group, (2) only operation group (mice only underwent operation to exposed uterine horns from the side), (3) control group (mice underwent the same operation with the operation group + perfusion of lipopolysaccharide solution from uterine horns), and (4) administration group (mice underwent the same operation with the control group + oral administration from days 0 to 9). was administered via oral gavage. On day 10, we investigated protein expression, uterine microbiome, and lipid metabolism in uterine tissues. Consequently, orally administered altered the uterine microbiome and induced proliferation of and species. The effects can contribute to show the anti-inflammatory effect through the interferon-β upregulation in uterine tissues. Additionally, oral administration resulted in the upregulations of some lipid metabolites, such as ω-3 polyunsaturated fatty acid resolvin D5, in uterine tissues, and resolvin D5 showed anti-inflammatory effects. However, the orally administered induced anti-inflammatory effect was attenuated with the deletion of G protein-coupled receptor 120 and 15-lipooxgenase inhibition. In conclusion, in the gut has anti-inflammatory effects on uterine tissues through alterations in the uterine microbiome and lipid metabolism. This study revealed a gut-uterus axis mechanism and provided insights into the treatment and prophylaxis of endometritis.