Effect of blood components, abdominal distension, and ecdysone therapy on the ultrastructural organization of posterior midgut epithelial cells and perimicrovillar membranes in Rhodnius prolixus

• Published in: Memórias do Instituto Oswaldo Cruz 2004-12, Vol.99 (8), p.815-822
• Main Authors: Albuquerque-Cunha, J M, Mello, C B, Garcia, E S, Azambuja, P, Souza, W de, Gonzalez, M S, Nogueira, N F S
• Format: Article
• Language: English
• Subjects: Animals; Blood; Ecdysone - pharmacology; hormone; Intestines - drug effects; Intestines - growth & development; Intestines - ultrastructure; Male; Microscopy, Electron, Transmission; microvilli; Microvilli - drug effects; Microvilli - ultrastructure; midgut; Nymph - drug effects; Nymph - growth & development; Nymph - ultrastructure; PARASITOLOGY; perimicrovillar membranes; Reduviidae; Rhodnius - drug effects; Rhodnius - ultrastructure; Rhodnius prolixus; triatomine; TROPICAL MEDICINE
• ISSN: 0074-0276; 1678-8060; 0074-0276; 1678-8060
• DOI: 10.1590/S0074-02762004000800006

The effects of blood components, nerve-cord severance, and ecdysone therapy on the posterior midgut epithelial cells of 5th-instar Rhodnius prolixus nymphs 10 days after feeding were analyzed by transmission electron microscopy. Cutting the nerve-cord of the blood-fed insects partially reduced the development of microvilli and perimicrovillar membranes (PMM), and produced large vacuoles and small electrondense granules; insects fed on Ringer's saline diet exhibited well developed microvilli and low PMM production; swolled rough endoplasmatic reticulum and electrondense granules; Ringer's saline meal with ecdysone led to PMM development, glycogen particles, and several mitochondria in the cytoplasm; epithelial cells of the insects fed on Ringer's saline meal whose nerve-cord was severed showed heterogeneously distributed microvilli with reduced PMM production and a great quantity of mitochondria and glycogen in the cytoplasm; well developed microvilli and PMM were observed in nerve-cord severed insects fed on Ringer's saline meal with ecdysone; Ringer's saline diet containing hemoglobin recovered the release of PMM; and insects fed on human plasma showed slightly reduced PMM production, although the addition of ecdysone in the plasma led to a normal midgut ultrastructural organization. We suggest that the full development of microvilli and PMM in the epithelial cells depends on the abdominal distension in addition to ingestion of hemoglobin, and the release of ecdysone.