Anti-tumor effect of Wasabi component, 6-(methylsulfinyl) hexyl isothiocyanate, against endometrial carcinoma cells

Purpose Wasabi is a traditional plant seasoning with an anti-septic function. Recent studies revealed several functions of Wasabi, such as anti-inflammation; however, the anti-tumor effect against endometrial carcinoma (EMC) cells has not been examined. In the present study, we investigated the anti...

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Published in:Discover. Oncology 2023-01, Vol.14 (1), p.9-9, Article 9
Main Authors: Ono, Motoki, Miyamoto, Tsutomu, Fuseya, Chiho, Asaka, Ryoichi, Ando, Hirofumi, Tanaka, Yasuhiro, Shinagawa, Manaka, Yokokawa, Yusuke, Takeuchi, Hodaka, Horiuchi, Akiko, Shiozawa, Tanri
Format: Article
Language:English
Subjects:
6-(Methylsulfinyl) hexyl isothiocyanate
Anti-tumor effect
Antibodies
Apoptosis
Cancer Research
Cells
Cytotoxicity
Endometrial cancer
Endometrial carcinoma
Experiments
Internal Medicine
Medicine
Medicine & Public Health
Molecular Medicine
Natural killer cell activity
Oncology
Oral administration
Radiotherapy
Reagents
Spleen
Surgical Oncology
Tumors
Wasabi
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Summary:Purpose Wasabi is a traditional plant seasoning with an anti-septic function. Recent studies revealed several functions of Wasabi, such as anti-inflammation; however, the anti-tumor effect against endometrial carcinoma (EMC) cells has not been examined. In the present study, we investigated the anti-tumor effect of 6-(methylsulfinyl) hexyl isothiocyanate (6-MITC), a major chemical compound of Wasabi, against various EMC cell lines in vitro and in vivo. Methods The effect of 6-MITC on cell viability was measured by the WST-1 assay in EMC and HUVEC cells. The impact of 6-MITC oral administration in nude mice was measured to assess the growth of the EMC xenograft and natural killer (NK) cell activity in the spleen. Results The addition of 6-MITC suppressed the proliferation of EMC cells (Ishikawa, HEC265, HEC108, KLE, and HEC1B) dose-dependently, but not HUVEC cells. 6-MITC (5 µM) enhanced the cisplatin sensitivity of EMC cells. 6-MITC induced apoptosis in a dose-dependent fashion in EMC cells other than HEC1B cells and was associated with increased expression of cleaved-caspase3 and decreased expression of BCL2. Oral administration of 6-MITC (2 and 4 µmol/kg) to Ishikawa and HEC1B xenografting mice resulted in a reduced tumor volume compared with the control (P 
ISSN:2730-6011
2730-6011
DOI:10.1007/s12672-023-00617-2