The Antigenicity of Epidemic SARS-CoV-2 Variants in the United Kingdom

To determine whether the neutralization activity of monoclonal antibodies, convalescent sera and vaccine-elicited sera was affected by the top five epidemic SARS-CoV-2 variants in the UK, including D614G+L18F+A222V, D614G+A222V, D614G+S477N, VOC-202012/01(B.1.1.7) and D614G+69-70del+N439K, a pseudov...

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Published in:Frontiers in immunology 2021-06, Vol.12, p.687869-687869
Main Authors: Wu, Jiajing, Zhang, Li, Zhang, Yue, Wang, Haixin, Ding, Ruxia, Nie, Jianhui, Li, Qianqian, Liu, Shuo, Yu, Yongxin, Yang, Xiaoming, Duan, Kai, Qu, Xiaowang, Wang, Youchun, Huang, Weijin
Format: Article
Language:English
Subjects:
B.1.1.7
Immunology
monoclonal antibody
mutation
neutralization
pseudotyped virus
vaccine
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Summary:To determine whether the neutralization activity of monoclonal antibodies, convalescent sera and vaccine-elicited sera was affected by the top five epidemic SARS-CoV-2 variants in the UK, including D614G+L18F+A222V, D614G+A222V, D614G+S477N, VOC-202012/01(B.1.1.7) and D614G+69-70del+N439K, a pseudovirus-neutralization assay was performed to evaluate the relative neutralization titers against the five SARS-CoV-2 variants and 12 single deconvolution mutants based on the variants. In this study, 18 monoclonal antibodies, 10 sera from convalescent COVID-19 patients, 10 inactivated-virus vaccine-elicited sera, 14 mRNA vaccine-elicited sera, nine RBD-immunized mouse sera, four RBD-immunized horse sera, and four spike-encoding DNA-immunized guinea pig sera were tested and analyzed. The N501Y, N439K, and S477N mutations caused immune escape from nine of 18 mAbs. However, the convalescent sera, inactivated virus vaccine-elicited sera, mRNA vaccine-elicited sera, spike DNA-elicited sera, and recombinant RBD protein-elicited sera could still neutralize these variants (within three-fold changes compared to the reference D614G variant). The neutralizing antibody responses to different types of vaccines were different, whereby the response to inactivated-virus vaccine was similar to the convalescent sera.
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2021.687869