In Vitro Anti-Inflammatory, Anticancer (MCF-7, 3T3, and HeLa Cell Lines), and Brine Shrimp Lethality Assay and FTIR Analysis of the Extract and Fractions of the Whole Plant of Heliotropium europaeum

In this study, anti-inflammatory, anticancer, brine shrimp lethality, and FTIR studies were evaluated. The oxidative burst assay using the chemiluminescence technique, MTT assay, brine shrimp lethality assay, and FTIR analysis were the methods used for the evaluation of anti-inflammatory, anticancer...

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Bibliographic Details
Published in:Mediators of inflammation 2020, Vol.2020 (2020), p.1-14
Main Authors: Imran, Muhammad, Panezai, Marina, Shahwani, Nisar Ahmed, Achakzai, Tehmina, Khan, Nazima Yousaf, Khan, Javed, Kakar, Abdul Manan, Akbar, Ali, Kakar, Shahabuddin, Akhtar, Basira, Anwar, Mohammad, Achakzai, Jahangir Khan, Khan, Ghulam Mustafa
Format: Article
Language:English
Subjects:
3T3 Cells
Alcohols
Alkanes
Amines
Analysis
Animals
Anti-inflammatory agents
Anti-Inflammatory Agents - chemistry
Anti-Inflammatory Agents - pharmacology
Anti-inflammatory drugs
Antineoplastic Agents - chemistry
Antineoplastic Agents - pharmacology
Aqueous solutions
Aromatic compounds
Artemia - drug effects
Butanol
Cancer
Carboxylic acids
Care and treatment
Cell lines
Chemiluminescence
Decapoda
Esters
HeLa Cells
Heliotropium - chemistry
Heliotropium europaeum
Herbal medicine
Humans
Inflammation
Lethality
MCF-7 Cells
Mice
n-Hexane
Phenols
Phosphoric acid
Plant extracts
Plant Extracts - chemistry
Plant Extracts - pharmacology
Plant sciences
Silane
Spectroscopy, Fourier Transform Infrared
Sulfate
Thiols
Toxicity
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Summary:In this study, anti-inflammatory, anticancer, brine shrimp lethality, and FTIR studies were evaluated. The oxidative burst assay using the chemiluminescence technique, MTT assay, brine shrimp lethality assay, and FTIR analysis were the methods used for the evaluation of anti-inflammatory, anticancer, brine shrimp lethality, and FTIR studies, respectively. The whole-plant butanol fraction of Heliotropium europaeum (WBFHE) showed anti-inflammatory activity on ROS having IC50 14.7±2.5 while the extract and other fractions of the whole plant of Heliotropium europaeum exhibited no anti-inflammatory activity. None of the extract and fractions of the whole plant of Heliotropium europaeum exhibited anticancer (MCF-7, 3T3, and HeLa cell lines) activities. The whole-plant aqueous fraction of Heliotropium europaeum (WAFHE) and whole-plant butanol fraction of Heliotropium europaeum (WBFHE) showed lethality at high concentration while at low concentration, no toxicity was shown. The whole-plant methanolic extract of Heliotropium europaeum (WMEHE) and whole-plant n-hexane fraction of Heliotropium europaeum (WHFHE) exhibited no toxicity. FTIR interpretation showed the functional groups for the aromatic compounds, phenols, carboxylic acids, esters, alkanes, alkenes, alcohols, alkyl halides, sulfate esters, phosphines, silanes, nitriles, thiols, amines, phosphoric acids, and nitro compounds.
ISSN:0962-9351
1466-1861
DOI:10.1155/2020/5056897