Influenza Virus-Like Particles Presenting both Toxoplasma gondii ROP4 and ROP13 Enhance Protection against T. gondii Infection

Rhoptry organelle proteins (ROPs) secreted by ( ) play a critical role during parasite invasion into host cells. In this study, virus-like particles (VLPs) vaccines containing ROP4 and/or ROP13 together with influenza M1 were generated. ROP4+ROP13 VLPs were produced by combining ROP4 VLPs with ROP13...

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Published in:Pharmaceutics 2019-07, Vol.11 (7), p.342
Main Authors: Kang, Hae-Ji, Lee, Su-Hwa, Kim, Min-Ju, Chu, Ki-Back, Lee, Dong-Hun, Chopra, Manika, Choi, Hyo-Jick, Park, Hyunwoo, Jin, Hui, Quan, Fu-Shi
Format: Article
Language:English
Subjects:
Antigens
Drug resistance
Feces
Infections
Influenza
Morphology
Polyclonal antibodies
protection
Proteins
Protozoa
Rodents
ROP13
ROP4
Toxoplasma gondii
vaccine
Vaccines
Vagina
virus-like particle
Viruses
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Summary:Rhoptry organelle proteins (ROPs) secreted by ( ) play a critical role during parasite invasion into host cells. In this study, virus-like particles (VLPs) vaccines containing ROP4 and/or ROP13 together with influenza M1 were generated. ROP4+ROP13 VLPs were produced by combining ROP4 VLPs with ROP13 VLPs, and ROP(4 + 13) VLPs by co-infecting insect cells with recombinant baculovirus expressing ROP4 or ROP13. Mice intranasally immunized with ROP(4 + 13) VLPs showed significantly higher levels of IgG, IgG1, IgG2a and IgA antibody responses in sera compared to ROP4+ROP13VLPs. Upon challenge infection by oral route, mice immunized with ROP(4 + 13) VLPs elicited higher levels of IgG and IgA antibody responses in fecal, urine, intestine and vaginal samples as well as CD4 T, CD8 T cells, and germinal center B cell responses compared to other type of vaccines, ROP4 VLPs, ROP13 VLPs, and ROP4+ROP13 VLPs. ROP(4 + 13) VLPs vaccination showed a significant decrease in the size and number of cyst in the brain and less body weight loss compared to combination ROP4+ROP13 VLPs upon challenge infection with ME49. These results indicated that the ROP(4 + 13) VLPs vaccination provided enhanced protection against infection compared to ROP4+ROP13 VLPs, providing an important insight into vaccine design strategy for VLPs vaccines.
ISSN:1999-4923
1999-4923
DOI:10.3390/pharmaceutics11070342