mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer

Colorectal cancer develops through two main genetic instability pathways characterized by distinct pathologic features and clinical outcome. We investigated colon cancer samples (23 characterized by microsatellite stability, MSS, and 16 by high microsatellite instability, MSI-H) for genome-wide expr...

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Bibliographic Details
Published in:Molecular cancer 2007-08, Vol.6 (1), p.54-54, Article 54
Main Authors: Lanza, Giovanni, Ferracin, Manuela, Gafà, Roberta, Veronese, Angelo, Spizzo, Riccardo, Pichiorri, Flavia, Liu, Chang-gong, Calin, George A, Croce, Carlo M, Negrini, Massimo
Format: Article
Language:English
Subjects:
Aged
Colorectal cancer
Colorectal Neoplasms - classification
Colorectal Neoplasms - genetics
Female
Gene Expression
Gene Expression Profiling
Genetic aspects
Health aspects
Humans
Immunohistochemistry
In Situ Hybridization
Male
Messenger RNA
MicroRNAs - analysis
Microsatellite Instability
Middle Aged
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
RNA, Neoplasm - genetics
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Summary:Colorectal cancer develops through two main genetic instability pathways characterized by distinct pathologic features and clinical outcome. We investigated colon cancer samples (23 characterized by microsatellite stability, MSS, and 16 by high microsatellite instability, MSI-H) for genome-wide expression of microRNA (miRNA) and mRNA. Based on combined miRNA and mRNA gene expression, a molecular signature consisting of twenty seven differentially expressed genes, inclusive of 8 miRNAs, could correctly distinguish MSI-H versus MSS colon cancer samples. Among the differentially expressed miRNAs, various members of the oncogenic miR-17-92 family were significantly up-regulated in MSS cancers. The majority of protein coding genes were also up-regulated in MSS cancers. Their functional classification revealed that they were most frequently associated with cell cycle, DNA replication, recombination, repair, gastrointestinal disease and immune response. This is the first report that indicates the existence of differences in miRNA expression between MSS versus MSI-H colorectal cancers. In addition, the work suggests that the combination of mRNA/miRNA expression signatures may represent a general approach for improving bio-molecular classification of human cancer.
ISSN:1476-4598
1476-4598
DOI:10.1186/1476-4598-6-54