Identification of Differential N-Glycan Compositions in the Serum and Tissue of Colon Cancer Patients by Mass Spectrometry

Colorectal cancer (CRC) ranks second as the leading cause of cancer-related deaths worldwide. N-glycosylation is one of the most common posttranslational protein modifications. Therefore, we studied the total serum N-glycome (TSNG) of 13 colon cancer patients compared to healthy controls using MALDI...

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Published in:Biology (Basel, Switzerland) Switzerland), 2021-04, Vol.10 (4), p.343
Main Authors: Coura, Marcelo de M A, Barbosa, Eder A, Brand, Guilherme D, Bloch, Jr, Carlos, de Sousa, Joao B
Format: Article
Language:English
Subjects:
Biomarkers
Chromatography
Colon cancer
Colonoscopy
Colorectal cancer
Colorectal carcinoma
Glycoproteins
Glycosylation
Inflammatory bowel disease
LC/MS
MALDI-TOF/MS
Mannose
Mass spectrometry
Mass spectroscopy
Medical prognosis
Mutation
N-glycans
N-glycosylation
Polysaccharides
Protein expression
Proteins
Scientific imaging
Tumor microenvironment
Tumors
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Summary:Colorectal cancer (CRC) ranks second as the leading cause of cancer-related deaths worldwide. N-glycosylation is one of the most common posttranslational protein modifications. Therefore, we studied the total serum N-glycome (TSNG) of 13 colon cancer patients compared to healthy controls using MALDI-TOF/MS and LC-MS. N-glycosylation of cancer tumor samples from the same cohort were further quantified using a similar methodology. In total, 23 N-glycan compositions were down-regulated in the serum of colon cancer patients, mostly galactosylated forms whilst the mannose-rich HexNAc2Hex7, the fucosylated bi-antennary glycan HexNAc4Hex5Fuc1NeuAc2, and the tetra-antennary HexNAc6Hex7NeuAc3 were up-regulated in serum. Hierarchical clustering analysis of TSNG correctly singled out 85% of the patients from controls. Albeit heterogenous, N-glycosylation of tumor samples showed overrepresented oligomannosidic, bi-antennary hypogalactosylated, and branched compositions related to normal colonic tissue, in both MALDI-TOF/MS and LC-MS analysis. Moreover, compositions found upregulated in tumor tissue were mostly uncorrelated to compositions in serum of cancer patients. Mass spectrometry-based N-glycan profiling in serum shows potential in the discrimination of patients from healthy controls. However, the compositions profile in serum showed no parallel with N-glycans in tumor microenvironment, which suggests a different origin of compositions found in serum of cancer patients.
ISSN:2079-7737
2079-7737
DOI:10.3390/biology10040343