Effect of alum co-adjuvantation of oil adjuvant vaccine on emulsion stability and immune responses against haemorhagic septicaemia in mice

Haemorrhagic septicaemia (HS), caused by Pasteurella multocida, is the most important bacterial disease of cattle and buffaloes in India. Oil adjuvant vaccine (OAV) is the most potent vaccine available for the control of HS. The study aims to evaluate the effect of alum co-adjuvantation of OAV on em...

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Bibliographic Details
Published in:Iranian journal of microbiology 2015-04, Vol.7 (2), p.79-87
Main Authors: Kumar, Sujeet, Chaturvedi, Vinod Kumar, Kumar, Bablu, Kumar, Pankaj, Somarajan, Sudha Rani, Mishra, Anil Kumar, Sharma, Bhaskar
Format: Article
Language:English
Subjects:
Alum
Emulsion stability
Haemorrhagic septicaemia
Immune response
Oil adjuvant vaccine
Original
Pasteurella multocida
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Summary:Haemorrhagic septicaemia (HS), caused by Pasteurella multocida, is the most important bacterial disease of cattle and buffaloes in India. Oil adjuvant vaccine (OAV) is the most potent vaccine available for the control of HS. The study aims to evaluate the effect of alum co-adjuvantation of OAV on emulsion stability and immune response. Two different oil adjuvant vaccines viz., standard oil adjuvant vaccine (OAV) and alum precipitated oil adjuvant vaccine (A-OAV) were prepared with Pasteurella multocida antigen. Emulsion stability was tested by centrifugation, storage at 37 °C for 3 months and microscopy. Immune responses were evaluated by ELISA antibody titer, CD4, CD8 T cell populations and survival post challenge by P. multocida in mice. The separation of aqueous and oil phase of emulsion by centrifugation and storage test were 0 and 6.76% in A-OAV as compared to 11.00 and 26.39% in OAV, respectively. The mean droplet size was significantly smaller (p
ISSN:2008-3289
2008-4447