A Haspin promoter element induces tissue-specific methylation of a transcription region and the regulation of gene expression in mouse ova

HASPIN is a nuclear serine-threonine kinase originally identified in the mouse testis. Its 193 bp DNA promoter element (hereafter, 193PE) regulates bidirectional, synchronous gene expression in the germ cells of male mice. Recent studies have shown that Haspin is also expressed in trace amounts in s...

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Bibliographic Details
Published in:Cell journal (Yakhteh) 2022-09, Vol.24 (9), p.552-554
Main Authors: Tanaka, Hiromitsu, Tokuhiro, Keizo
Format: Article
Language:English
Subjects:
Deoxyribonucleic acid
DNA
DNA methylation
embryo
Gametocytes
Gene expression
Gene regulation
germ cell
Germ cells
haspin
Hepatocytes
inner cell mass
Kinases
oocyte
Oocytes
Ovulation
Pituitary (anterior)
Protein-serine/threonine kinase
Reporter gene
Somatic cells
Tissues
Transgenic animals
Transgenic mice
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Summary:HASPIN is a nuclear serine-threonine kinase originally identified in the mouse testis. Its 193 bp DNA promoter element (hereafter, 193PE) regulates bidirectional, synchronous gene expression in the germ cells of male mice. Recent studies have shown that Haspin is also expressed in trace amounts in somatic cells; HASPIN also functions in oocytes. Haspin expression is regulated by the tissue-specific methylation of Haspin genomic DNA regions, including somatic cells. This study investigated relationship between 193PE and DNA methylation by examining methylation status of transgenic mice carrying 193PE and a reporter gene. In somatic (liver) cells carrying the reporter gene, 193PE induced methylation as well as trace expression of the reporter gene. In the testis, 193PE induced hypomethylation and intense reporter gene expression. Expression of HASPIN in an egg was assessed using human chorionic gonadotrophin to induce ovulation in female transgenic mice. The results showed that 193PE induced tissue-specific methylation, which resulted in reporter gene expression in a mouse egg.
ISSN:2228-5806
2228-5814
DOI:10.22074/cellj.2022.8444