Detection of Hepatitis E Virus in Shellfish Harvesting Areas from Galicia (Northwestern Spain)

The hepatitis E virus (HEV) affects almost 20 million individuals annually, causing approximately 3.3 million acute liver injuries, 56,600 deaths, and huge healthcare-associated economic losses. Shellfish produced close to urban and livestock areas can bioaccumulate this virus and transmit it to the...

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Bibliographic Details
Published in:Viruses 2019-07, Vol.11 (7), p.618
Main Authors: Rivadulla, Enrique, Varela, Miguel F, Mesquita, João R, Nascimento, Maria S J, Romalde, Jesús L
Format: Article
Language:English
Subjects:
Animals
bioaccumulation
Bivalvia
Bivalvia - virology
detection
E coli
financial economics
Food contamination & poisoning
Food Microbiology
food safety
genome
Genomes
Genotype
Genotypes
Genotyping
harvesting
Hepatitis
hepatitis E virus
Hepatitis E virus - classification
Hepatitis E virus - genetics
Hepatitis E virus - isolation & purification
human population
Humans
liver
Livestock
Mollusks
Open reading frames
Orthohepevirus A
Phylogeny
Prevalence
Proteins
quantitative polymerase chain reaction
reverse transcriptase polymerase chain reaction
Reverse transcription
RNA
RNA, Viral - genetics
sequence analysis
Sequence Analysis, RNA
Shellfish
Shellfish - virology
Spain
Spain - epidemiology
subgenotype
swine
Thermal cycling
Viruses
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Summary:The hepatitis E virus (HEV) affects almost 20 million individuals annually, causing approximately 3.3 million acute liver injuries, 56,600 deaths, and huge healthcare-associated economic losses. Shellfish produced close to urban and livestock areas can bioaccumulate this virus and transmit it to the human population. The aim of this study was to evaluate the presence of HEV in molluscan shellfish, in order to deepen the knowledge about HEV prevalence in Galicia (northwestern Spain), and to investigate this as a possible route of HEV transmission to humans. A total of 168 shellfish samples was obtained from two different Galician rías (Ría de Ares-Betanzos and Ría de Vigo). The samples were analyzed by reverse transcription-quantitative PCR (RT-qPCR). RT-nested PCR and sequencing were used for further genotyping and phylogenetic analysis of positive samples. HEV was detected in 41 (24.4%) samples, at quantification levels ranging from non-quantifiable (
ISSN:1999-4915
1999-4915
DOI:10.3390/v11070618