Comparison of High-Performance Liquid Chromatography and Capillary Electrophoresis in the Screening of Haemoglobinopathies

Objective: To compare the results of High-Performance Liquid Chromatography and Capillary Electrophoresis in the screening of haemoglobinopathies. Study Design: Cross-sectional study. Place and Duration of Study: Department of Haematology, Armed Forces Institute of Pathology, Rawalpindi Pakistan, fr...

Full description

Saved in:
Bibliographic Details
Published in:Pakistan Armed Forces medical journal 2024-04, Vol.74 (2), p.357-361
Main Authors: Asghar, Shumaila, Mahmood Abbasi, Asad, Zafar, Jawad, Mahmood, Rafia, Zahir, Saima, Khan, Maria
Format: Article
Language:English
Subjects:
Analysis
Automation
Bar codes
Beta-thalassaemia
Blood diseases
Capillary electrophoresis
Chromatography
Comparative analysis
Cross-sectional studies
Electrophoresis
Ethylenediaminetetraacetic acid
Haemoglobinopathies
Hemoglobin
High performance liquid chromatography
Malaria
Medical diagnosis
Medical screening
Methods
Sickle cell anemia
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 361
container_issue 2
container_start_page 357
container_title Pakistan Armed Forces medical journal
container_volume 74
creator Asghar, Shumaila
Mahmood Abbasi, Asad
Zafar, Jawad
Mahmood, Rafia
Zahir, Saima
Khan, Maria
description Objective: To compare the results of High-Performance Liquid Chromatography and Capillary Electrophoresis in the screening of haemoglobinopathies. Study Design: Cross-sectional study. Place and Duration of Study: Department of Haematology, Armed Forces Institute of Pathology, Rawalpindi Pakistan, from May 2020 to Nov 2020. Methodology: Ninety(90) newly diagnosed patients with haemoglobinopathies were included. Patients with a history of recent blood transfusion (
doi_str_mv 10.51253/pafmj.v74i2.7452
format article
fullrecord <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_1d0778634ea74b36817718812a630a21</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A792890740</galeid><doaj_id>oai_doaj_org_article_1d0778634ea74b36817718812a630a21</doaj_id><sourcerecordid>A792890740</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3162-d22bb4f6c69a5f5faeb921206fdc0ecce2725592c8d7b82027ec6cfca961c1703</originalsourceid><addsrcrecordid>eNptkkFr3DAQhU1poUuSH9CboWdvpZFkycewpE1gIYW0ZzGWJVuLLTmSt5D--jre0lLo6DDwmPfxBK8oPlCyFxQE-zSjm077H5J72Esu4E2xA05ppRSHt8WOEEaqpubqfXGT84msIxhwInbFz0OcZkw-x1BGV977fqi-2uRimjAYWx7989l35WFIccIl9gnn4aXEsEo4-3HE9FLejdYsKc5DTDb7XPpQLoMtn0yyNvjQb2C0U-zH2PoQZ1wGb_N18c7hmO3N731VfP989-1wXx0fvzwcbo-VYbSGqgNoW-5qUzconHBo2wYokNp1hlhjLEgQogGjOtkqICCtqY0z2NTUUEnYVfFw4XYRT3pOflpD64heb0JMvca0eDNaTTsipaoZtyh5y2pFpaRKUcCaEQS6sj5eWHOKz2ebF32K5xTW-JoRAUI1q-PvVY8r1AcXl4Rm8tnoW9mAaojkr7n2_7laX2cnb2Kwzq_6PwZ6MZgUc07W_fkMJXorgt6KoLci6NcisF9r06da</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3052589177</pqid></control><display><type>article</type><title>Comparison of High-Performance Liquid Chromatography and Capillary Electrophoresis in the Screening of Haemoglobinopathies</title><source>Nexis UK</source><source>Publicly Available Content (ProQuest)</source><creator>Asghar, Shumaila ; Mahmood Abbasi, Asad ; Zafar, Jawad ; Mahmood, Rafia ; Zahir, Saima ; Khan, Maria</creator><creatorcontrib>Asghar, Shumaila ; Mahmood Abbasi, Asad ; Zafar, Jawad ; Mahmood, Rafia ; Zahir, Saima ; Khan, Maria</creatorcontrib><description>Objective: To compare the results of High-Performance Liquid Chromatography and Capillary Electrophoresis in the screening of haemoglobinopathies. Study Design: Cross-sectional study. Place and Duration of Study: Department of Haematology, Armed Forces Institute of Pathology, Rawalpindi Pakistan, from May 2020 to Nov 2020. Methodology: Ninety(90) newly diagnosed patients with haemoglobinopathies were included. Patients with a history of recent blood transfusion (&lt;4 weeks) were excluded. Venous blood samples were taken in EDTA tubes, separated into two aliquots and evaluated in parallel on High-Performance Liquid Chromatography and Capillary Electrophoresis analyzers. Results: Nine different haemoglobinopathies were encountered in the study sample. The most common condition was beta thalassaemia trait found in 50(55.6%) subjects, while Haemoglobin (Hb) (D) homozygous was the least common, detected in only 1(1.1%) subjects. A significant difference was observed between values in cases of Hb(D) Iran trait; Mean Hb(A2) percentage detected by HPLC was 41.96±1.46% compared to 1.5±0.79% detected by CE (p-value&lt;0.001). CE detected a mean of 41.4±1.01% of Hb(D)Iran in the patients of the Hb(D) Iran trait, while High-Performance Liquid Chromatography did not detect this Hb variant (p-value&lt;0.001). A significant difference was also found in cases of Hb(E) trait in percentages of Hb(A2) and Hb(E) variants. High-Performance Liquid Chromatography detected a mean of 31.43±2.84% of Hb(A2) compared to 4.5±0.5% detected by CE (p-value=0.003). Conclusion: Capillary electrophoresis is capable of identifying haemoglobin variants Hb(D) Iran and Hb(E) in cases of Hb(D) Iran trait and Hb(E) trait/Hb(E) homozygous, which HPLC does not pick up.</description><identifier>ISSN: 0030-9648</identifier><identifier>EISSN: 2411-8842</identifier><identifier>DOI: 10.51253/pafmj.v74i2.7452</identifier><language>eng</language><publisher>Rawalpindi: Knowledge Bylanes</publisher><subject>Analysis ; Automation ; Bar codes ; Beta-thalassaemia ; Blood diseases ; Capillary electrophoresis ; Chromatography ; Comparative analysis ; Cross-sectional studies ; Electrophoresis ; Ethylenediaminetetraacetic acid ; Haemoglobinopathies ; Hemoglobin ; High performance liquid chromatography ; Malaria ; Medical diagnosis ; Medical screening ; Methods ; Sickle cell anemia</subject><ispartof>Pakistan Armed Forces medical journal, 2024-04, Vol.74 (2), p.357-361</ispartof><rights>COPYRIGHT 2024 Knowledge Bylanes</rights><rights>(c)2024 Pakistan Armed Forces Medical Journal</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3052589177/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3052589177?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,25753,27924,27925,37012,44590,74998</link.rule.ids></links><search><creatorcontrib>Asghar, Shumaila</creatorcontrib><creatorcontrib>Mahmood Abbasi, Asad</creatorcontrib><creatorcontrib>Zafar, Jawad</creatorcontrib><creatorcontrib>Mahmood, Rafia</creatorcontrib><creatorcontrib>Zahir, Saima</creatorcontrib><creatorcontrib>Khan, Maria</creatorcontrib><title>Comparison of High-Performance Liquid Chromatography and Capillary Electrophoresis in the Screening of Haemoglobinopathies</title><title>Pakistan Armed Forces medical journal</title><description>Objective: To compare the results of High-Performance Liquid Chromatography and Capillary Electrophoresis in the screening of haemoglobinopathies. Study Design: Cross-sectional study. Place and Duration of Study: Department of Haematology, Armed Forces Institute of Pathology, Rawalpindi Pakistan, from May 2020 to Nov 2020. Methodology: Ninety(90) newly diagnosed patients with haemoglobinopathies were included. Patients with a history of recent blood transfusion (&lt;4 weeks) were excluded. Venous blood samples were taken in EDTA tubes, separated into two aliquots and evaluated in parallel on High-Performance Liquid Chromatography and Capillary Electrophoresis analyzers. Results: Nine different haemoglobinopathies were encountered in the study sample. The most common condition was beta thalassaemia trait found in 50(55.6%) subjects, while Haemoglobin (Hb) (D) homozygous was the least common, detected in only 1(1.1%) subjects. A significant difference was observed between values in cases of Hb(D) Iran trait; Mean Hb(A2) percentage detected by HPLC was 41.96±1.46% compared to 1.5±0.79% detected by CE (p-value&lt;0.001). CE detected a mean of 41.4±1.01% of Hb(D)Iran in the patients of the Hb(D) Iran trait, while High-Performance Liquid Chromatography did not detect this Hb variant (p-value&lt;0.001). A significant difference was also found in cases of Hb(E) trait in percentages of Hb(A2) and Hb(E) variants. High-Performance Liquid Chromatography detected a mean of 31.43±2.84% of Hb(A2) compared to 4.5±0.5% detected by CE (p-value=0.003). Conclusion: Capillary electrophoresis is capable of identifying haemoglobin variants Hb(D) Iran and Hb(E) in cases of Hb(D) Iran trait and Hb(E) trait/Hb(E) homozygous, which HPLC does not pick up.</description><subject>Analysis</subject><subject>Automation</subject><subject>Bar codes</subject><subject>Beta-thalassaemia</subject><subject>Blood diseases</subject><subject>Capillary electrophoresis</subject><subject>Chromatography</subject><subject>Comparative analysis</subject><subject>Cross-sectional studies</subject><subject>Electrophoresis</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Haemoglobinopathies</subject><subject>Hemoglobin</subject><subject>High performance liquid chromatography</subject><subject>Malaria</subject><subject>Medical diagnosis</subject><subject>Medical screening</subject><subject>Methods</subject><subject>Sickle cell anemia</subject><issn>0030-9648</issn><issn>2411-8842</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptkkFr3DAQhU1poUuSH9CboWdvpZFkycewpE1gIYW0ZzGWJVuLLTmSt5D--jre0lLo6DDwmPfxBK8oPlCyFxQE-zSjm077H5J72Esu4E2xA05ppRSHt8WOEEaqpubqfXGT84msIxhwInbFz0OcZkw-x1BGV977fqi-2uRimjAYWx7989l35WFIccIl9gnn4aXEsEo4-3HE9FLejdYsKc5DTDb7XPpQLoMtn0yyNvjQb2C0U-zH2PoQZ1wGb_N18c7hmO3N731VfP989-1wXx0fvzwcbo-VYbSGqgNoW-5qUzconHBo2wYokNp1hlhjLEgQogGjOtkqICCtqY0z2NTUUEnYVfFw4XYRT3pOflpD64heb0JMvca0eDNaTTsipaoZtyh5y2pFpaRKUcCaEQS6sj5eWHOKz2ebF32K5xTW-JoRAUI1q-PvVY8r1AcXl4Rm8tnoW9mAaojkr7n2_7laX2cnb2Kwzq_6PwZ6MZgUc07W_fkMJXorgt6KoLci6NcisF9r06da</recordid><startdate>20240430</startdate><enddate>20240430</enddate><creator>Asghar, Shumaila</creator><creator>Mahmood Abbasi, Asad</creator><creator>Zafar, Jawad</creator><creator>Mahmood, Rafia</creator><creator>Zahir, Saima</creator><creator>Khan, Maria</creator><general>Knowledge Bylanes</general><general>AsiaNet Pakistan (Pvt) Ltd</general><general>Army Medical College Rawalpindi</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>DOA</scope></search><sort><creationdate>20240430</creationdate><title>Comparison of High-Performance Liquid Chromatography and Capillary Electrophoresis in the Screening of Haemoglobinopathies</title><author>Asghar, Shumaila ; Mahmood Abbasi, Asad ; Zafar, Jawad ; Mahmood, Rafia ; Zahir, Saima ; Khan, Maria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3162-d22bb4f6c69a5f5faeb921206fdc0ecce2725592c8d7b82027ec6cfca961c1703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Analysis</topic><topic>Automation</topic><topic>Bar codes</topic><topic>Beta-thalassaemia</topic><topic>Blood diseases</topic><topic>Capillary electrophoresis</topic><topic>Chromatography</topic><topic>Comparative analysis</topic><topic>Cross-sectional studies</topic><topic>Electrophoresis</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Haemoglobinopathies</topic><topic>Hemoglobin</topic><topic>High performance liquid chromatography</topic><topic>Malaria</topic><topic>Medical diagnosis</topic><topic>Medical screening</topic><topic>Methods</topic><topic>Sickle cell anemia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Asghar, Shumaila</creatorcontrib><creatorcontrib>Mahmood Abbasi, Asad</creatorcontrib><creatorcontrib>Zafar, Jawad</creatorcontrib><creatorcontrib>Mahmood, Rafia</creatorcontrib><creatorcontrib>Zahir, Saima</creatorcontrib><creatorcontrib>Khan, Maria</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Pakistan Armed Forces medical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Asghar, Shumaila</au><au>Mahmood Abbasi, Asad</au><au>Zafar, Jawad</au><au>Mahmood, Rafia</au><au>Zahir, Saima</au><au>Khan, Maria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of High-Performance Liquid Chromatography and Capillary Electrophoresis in the Screening of Haemoglobinopathies</atitle><jtitle>Pakistan Armed Forces medical journal</jtitle><date>2024-04-30</date><risdate>2024</risdate><volume>74</volume><issue>2</issue><spage>357</spage><epage>361</epage><pages>357-361</pages><issn>0030-9648</issn><eissn>2411-8842</eissn><abstract>Objective: To compare the results of High-Performance Liquid Chromatography and Capillary Electrophoresis in the screening of haemoglobinopathies. Study Design: Cross-sectional study. Place and Duration of Study: Department of Haematology, Armed Forces Institute of Pathology, Rawalpindi Pakistan, from May 2020 to Nov 2020. Methodology: Ninety(90) newly diagnosed patients with haemoglobinopathies were included. Patients with a history of recent blood transfusion (&lt;4 weeks) were excluded. Venous blood samples were taken in EDTA tubes, separated into two aliquots and evaluated in parallel on High-Performance Liquid Chromatography and Capillary Electrophoresis analyzers. Results: Nine different haemoglobinopathies were encountered in the study sample. The most common condition was beta thalassaemia trait found in 50(55.6%) subjects, while Haemoglobin (Hb) (D) homozygous was the least common, detected in only 1(1.1%) subjects. A significant difference was observed between values in cases of Hb(D) Iran trait; Mean Hb(A2) percentage detected by HPLC was 41.96±1.46% compared to 1.5±0.79% detected by CE (p-value&lt;0.001). CE detected a mean of 41.4±1.01% of Hb(D)Iran in the patients of the Hb(D) Iran trait, while High-Performance Liquid Chromatography did not detect this Hb variant (p-value&lt;0.001). A significant difference was also found in cases of Hb(E) trait in percentages of Hb(A2) and Hb(E) variants. High-Performance Liquid Chromatography detected a mean of 31.43±2.84% of Hb(A2) compared to 4.5±0.5% detected by CE (p-value=0.003). Conclusion: Capillary electrophoresis is capable of identifying haemoglobin variants Hb(D) Iran and Hb(E) in cases of Hb(D) Iran trait and Hb(E) trait/Hb(E) homozygous, which HPLC does not pick up.</abstract><cop>Rawalpindi</cop><pub>Knowledge Bylanes</pub><doi>10.51253/pafmj.v74i2.7452</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0030-9648
ispartof Pakistan Armed Forces medical journal, 2024-04, Vol.74 (2), p.357-361
issn 0030-9648
2411-8842
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_1d0778634ea74b36817718812a630a21
source Nexis UK; Publicly Available Content (ProQuest)
subjects Analysis
Automation
Bar codes
Beta-thalassaemia
Blood diseases
Capillary electrophoresis
Chromatography
Comparative analysis
Cross-sectional studies
Electrophoresis
Ethylenediaminetetraacetic acid
Haemoglobinopathies
Hemoglobin
High performance liquid chromatography
Malaria
Medical diagnosis
Medical screening
Methods
Sickle cell anemia
title Comparison of High-Performance Liquid Chromatography and Capillary Electrophoresis in the Screening of Haemoglobinopathies
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T00%3A33%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20High-Performance%20Liquid%20Chromatography%20and%20Capillary%20Electrophoresis%20in%20the%20Screening%20of%20Haemoglobinopathies&rft.jtitle=Pakistan%20Armed%20Forces%20medical%20journal&rft.au=Asghar,%20Shumaila&rft.date=2024-04-30&rft.volume=74&rft.issue=2&rft.spage=357&rft.epage=361&rft.pages=357-361&rft.issn=0030-9648&rft.eissn=2411-8842&rft_id=info:doi/10.51253/pafmj.v74i2.7452&rft_dat=%3Cgale_doaj_%3EA792890740%3C/gale_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c3162-d22bb4f6c69a5f5faeb921206fdc0ecce2725592c8d7b82027ec6cfca961c1703%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=3052589177&rft_id=info:pmid/&rft_galeid=A792890740&rfr_iscdi=true