Generating Functional and Highly Proliferative Melanocytes Derived from Human Pluripotent Stem Cells: A Promising Tool for Biotherapeutic Approaches to Treat Skin Pigmentation Disorders

Melanocytes are essential for skin homeostasis and protection, and their loss or misfunction leads to a wide spectrum of diseases. Cell therapy utilizing autologous melanocytes has been used for years as an adjunct treatment for hypopigmentary disorders such as vitiligo. However, these approaches ar...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-03, Vol.24 (7), p.6398
Main Authors: Saidani, Manoubia, Darle, Annabelle, Jarrige, Margot, Polveche, Hélène, El Kassar, Lina, Julié, Séverine, Bessou-Touya, Sandrine, Holic, Nathalie, Lemaitre, Gilles, Martinat, Cécile, Baldeschi, Christine, Allouche, Jennifer
Format: Article
Language:English
Subjects:
Biopsy
Cell culture
Cell therapy
Flow cytometry
Gene expression
Genetic transformation
Genomes
Homeostasis
human pluripotent stem cell
Humans
Life Sciences
Localization
Melanocytes
Melanocytes - pathology
Melanoma
Methods
Morphology
Mutation
Pigmentation
Pigmentation disorders
Pigmentation Disorders - therapy
pigmentation-related skin disorders
Pluripotency
Pluripotent Stem Cells
Protocol
Skin
Skin - pathology
Skin Pigmentation
skin regeneration
Stem cells
Transcription factors
Transcriptomics
Vitiligo
Vitiligo - pathology
Vitiligo - therapy
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Summary:Melanocytes are essential for skin homeostasis and protection, and their loss or misfunction leads to a wide spectrum of diseases. Cell therapy utilizing autologous melanocytes has been used for years as an adjunct treatment for hypopigmentary disorders such as vitiligo. However, these approaches are hindered by the poor proliferative capacity of melanocytes obtained from skin biopsies. Recent advances in the field of human pluripotent stem cells have fueled the prospect of generating melanocytes. Here, we have developed a well-characterized method to produce a pure and homogenous population of functional and proliferative melanocytes. The genetic stability and potential transformation of melanocytes from pluripotent stem cells have been evaluated over time during the in vitro culture process. Thanks to transcriptomic analysis, the molecular signatures all along the differentiation protocol have been characterized, providing a solid basis for standardizing the protocol. Altogether, our results promise meaningful, broadly applicable, and longer-lasting advances for pigmentation disorders and open perspectives for innovative biotherapies for pigment disorders.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24076398