Hydrophilic Interaction Liquid Chromatography-Electrospray Ionization Mass Spectrometry for Therapeutic Drug Monitoring of Metformin and Rosuvastatin in Human Plasma

In this work a hydrophilic interaction liquid chromatography/positive ion electrospray mass spectrometric assay (HILIC/ESI-MS) has been developed and fully validated for the quantitation of metformin and rosuvastatin in human plasma. Sample preparation involved the use of 100 μL of human plasma, fol...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2018-06, Vol.23 (7), p.1548
Main Authors: Antonopoulos, Nikolaos, Machairas, Giorgos, Migias, George, Vonaparti, Ariadni, Brakoulia, Vasiliki, Pistos, Constantinos, Gennimata, Dimitra, Panderi, Irene
Format: Article
Language:English
Subjects:
Acetonitrile
Ammonium
Antidiabetics
Blood plasma
Chromatography
Chromatography, Liquid - methods
Electrospraying
Elution
Flow velocity
HILIC
Humans
Ionization
Ions
LC-MS
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Metformin
Metformin - blood
Plasma
Positive ions
Proteins
Quantitation
rosuvastatin
Rosuvastatin Calcium - blood
Sample preparation
Spectrometry, Mass, Electrospray Ionization - methods
Therapeutic drug monitoring
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Summary:In this work a hydrophilic interaction liquid chromatography/positive ion electrospray mass spectrometric assay (HILIC/ESI-MS) has been developed and fully validated for the quantitation of metformin and rosuvastatin in human plasma. Sample preparation involved the use of 100 μL of human plasma, following protein precipitation and filtration. Metformin, rosuvastatin and 4-[2-(propylamino) ethyl] indoline 2 one hydrochloride (internal standard) were separated by using an X-Bridge-HILIC BEH analytical column (150.0 × 2.1 mm i.d., particle size 3.5 μm) with isocratic elution. A mobile phase consisting of 12% ( / ) 15 mM ammonium formate water solution in acetonitrile was used for the separation and pumped at a flow rate of 0.25 mL min . The linear range of the assay was 100 to 5000 ng mL and 2 to 100 ng mL for metformin and rosuvastatin, respectively. The current HILIC-ESI/MS method allows for the accurate and precise quantitation of metformin and rosuvastatin in human plasma with a simple sample preparation and a short a chromatographic run time (less than 15 min). Plasma samples from eight patients were further analysed proving the capability of the proposed method to support a wide range of clinical studies.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules23071548