Accumulated in-situ spectral information analysis of room-temperature phosphorescence with time-gated bioimaging

This study introduces the time-gated analysis of room-temperature phosphorescence (RTP) for the in-situ analysis of the visible and spectral information of photons. Time-gated analysis is performed using a microscopic system consisting of a spectrometer, which is advantageous for in-situ analysis si...

Full description

Saved in:
Bibliographic Details
Published in:Materials today bio 2024-10, Vol.28, p.101238, Article 101238
Main Authors: Cho, Yong Ho, Kim, Seokho, Won, Tae Kyung, Cho, Sunki, Ahn, Dong June
Format: Article
Language:English
Subjects:
Autofluorescence
Full Length
In-situ spectroscopy
Room-temperature phosphorescence (RTP)
Signal accumulation
Time-gated microscopy
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study introduces the time-gated analysis of room-temperature phosphorescence (RTP) for the in-situ analysis of the visible and spectral information of photons. Time-gated analysis is performed using a microscopic system consisting of a spectrometer, which is advantageous for in-situ analysis since it facilitates the real-time measurement of luminescence signal changes. An RTP material hybridized with a DNA aptamer that targets a specific protein enhances the intensity and lifetime of phosphorescence after selective recognition with the target protein. In addition, time-gated analysis allows for the millisecond-scale imaging of phosphorescence signals, excluding autofluorescence, and improves the signal-to-background ratio (SBR) through the accumulation of signals. While collecting the time-gated images and spectra of RTP and autofluorescent materials simultaneously, we develop a method for obtaining phosphorescence signals by means of selective exclusion of autofluorescence signals in simulated or real cell conditions. It is confirmed that the accumulated time-gated analysis can provide ample information about luminescence signals for bioimaging and biosensing applications. [Display omitted] •The RTP materials are hybridized with DNA aptamer for bio-recognition avoiding autofluorescence with time-gated analyses.•A spectrometer is integrated with microscopy to obtain visible and spectral data, enhancing in-situ imaging and sensing analyses.•Time-gated analyses improve image and spectra by signal accumulation and noise reduction.
ISSN:2590-0064
2590-0064
DOI:10.1016/j.mtbio.2024.101238