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Polymerase chain reaction (PCR) for the detection of Salmonella in artificially inoculated chicken meat
The aim of this study was to develop a polymerase chain reaction (PCR) protocol for the detection of Salmonella in artificially contaminated chicken meat. Tests were performed with different dilutions of Salmonella Typhimurium or Salmonella Enteritidis cells (10(-7), 10(-8) or 10(-9) CFU/mL) inocula...
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| Published in: | Revista do Instituto de Medicina Tropical de São Paulo 2001-10, Vol.43 (5), p.247-250 |
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| cited_by | cdi_FETCH-LOGICAL-c4052-93bfc1c236d177217b1f5b46e042fd593f103b35bf0fdc49349381a7117193013 |
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| cites | cdi_FETCH-LOGICAL-c4052-93bfc1c236d177217b1f5b46e042fd593f103b35bf0fdc49349381a7117193013 |
| container_end_page | 250 |
| container_issue | 5 |
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| container_title | Revista do Instituto de Medicina Tropical de São Paulo |
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| creator | dos Santos, L R do Nascimento, V P de Oliveira, S D Flores, M L Pontes, A P Ribeiro, A R Salle, C T Lopes, R F |
| description | The aim of this study was to develop a polymerase chain reaction (PCR) protocol for the detection of Salmonella in artificially contaminated chicken meat. Tests were performed with different dilutions of Salmonella Typhimurium or Salmonella Enteritidis cells (10(-7), 10(-8) or 10(-9) CFU/mL) inoculated in chicken meat samples, in order to establish the limits of detection, incubation times (0, 6, 8 and 24 hours of pre-enrichment in PBW 1%) and three DNA extraction protocols (phenol-chloroform, thermal treatment and thermal treatment and Sephaglass). The assay was able to detect until 10(-9) CFU/mL of initial dilution of Salmonella cells inoculated in chicken meat, which allows detection of Salmonella within 48 hours, including 24 hours of pre-enrichment and using the phenol-chloroform DNA extraction protocol. As the results are obtained in a shorter time period than that of microbiological culture, this procedure will be useful in the methodology for detection of Salmonella in chicken. |
| doi_str_mv | 10.1590/s0036-46652001000500002 |
| format | article |
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Tests were performed with different dilutions of Salmonella Typhimurium or Salmonella Enteritidis cells (10(-7), 10(-8) or 10(-9) CFU/mL) inoculated in chicken meat samples, in order to establish the limits of detection, incubation times (0, 6, 8 and 24 hours of pre-enrichment in PBW 1%) and three DNA extraction protocols (phenol-chloroform, thermal treatment and thermal treatment and Sephaglass). The assay was able to detect until 10(-9) CFU/mL of initial dilution of Salmonella cells inoculated in chicken meat, which allows detection of Salmonella within 48 hours, including 24 hours of pre-enrichment and using the phenol-chloroform DNA extraction protocol. As the results are obtained in a shorter time period than that of microbiological culture, this procedure will be useful in the methodology for detection of Salmonella in chicken.</description><identifier>ISSN: 0036-4665</identifier><identifier>ISSN: 1678-9946</identifier><identifier>EISSN: 0036-4665</identifier><identifier>EISSN: 1678-9946</identifier><identifier>DOI: 10.1590/s0036-46652001000500002</identifier><identifier>PMID: 11696845</identifier><identifier>CODEN: RMTSAE</identifier><language>eng</language><publisher>Brazil: Instituto de Medicina Tropical de Sao Paulo</publisher><subject>Animals ; Chicken meat ; Chickens - microbiology ; DNA, Bacterial - analysis ; DNA, Bacterial - isolation & purification ; PCR ; Polymerase Chain Reaction ; Poultry Products - microbiology ; Salmonella ; Salmonella - isolation & purification ; Time Factors ; TROPICAL MEDICINE</subject><ispartof>Revista do Instituto de Medicina Tropical de São Paulo, 2001-10, Vol.43 (5), p.247-250</ispartof><rights>Copyright Instituto de Medicina Tropical de Sao Paulo Sep/Oct 2001</rights><rights>This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4052-93bfc1c236d177217b1f5b46e042fd593f103b35bf0fdc49349381a7117193013</citedby><cites>FETCH-LOGICAL-c4052-93bfc1c236d177217b1f5b46e042fd593f103b35bf0fdc49349381a7117193013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/196521034/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/196521034?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,24129,25731,27901,27902,36989,36990,44566,75096</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11696845$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>dos Santos, L R</creatorcontrib><creatorcontrib>do Nascimento, V P</creatorcontrib><creatorcontrib>de Oliveira, S D</creatorcontrib><creatorcontrib>Flores, M L</creatorcontrib><creatorcontrib>Pontes, A P</creatorcontrib><creatorcontrib>Ribeiro, A R</creatorcontrib><creatorcontrib>Salle, C T</creatorcontrib><creatorcontrib>Lopes, R F</creatorcontrib><title>Polymerase chain reaction (PCR) for the detection of Salmonella in artificially inoculated chicken meat</title><title>Revista do Instituto de Medicina Tropical de São Paulo</title><addtitle>Rev Inst Med Trop Sao Paulo</addtitle><description>The aim of this study was to develop a polymerase chain reaction (PCR) protocol for the detection of Salmonella in artificially contaminated chicken meat. Tests were performed with different dilutions of Salmonella Typhimurium or Salmonella Enteritidis cells (10(-7), 10(-8) or 10(-9) CFU/mL) inoculated in chicken meat samples, in order to establish the limits of detection, incubation times (0, 6, 8 and 24 hours of pre-enrichment in PBW 1%) and three DNA extraction protocols (phenol-chloroform, thermal treatment and thermal treatment and Sephaglass). The assay was able to detect until 10(-9) CFU/mL of initial dilution of Salmonella cells inoculated in chicken meat, which allows detection of Salmonella within 48 hours, including 24 hours of pre-enrichment and using the phenol-chloroform DNA extraction protocol. As the results are obtained in a shorter time period than that of microbiological culture, this procedure will be useful in the methodology for detection of Salmonella in chicken.</description><subject>Animals</subject><subject>Chicken meat</subject><subject>Chickens - microbiology</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>PCR</subject><subject>Polymerase Chain Reaction</subject><subject>Poultry Products - microbiology</subject><subject>Salmonella</subject><subject>Salmonella - isolation & purification</subject><subject>Time Factors</subject><subject>TROPICAL MEDICINE</subject><issn>0036-4665</issn><issn>1678-9946</issn><issn>0036-4665</issn><issn>1678-9946</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNp9UltvFCEYJUZjL_oXdOKDqQ9buc_yaDZemjSxsfpMGPhoWZmhwszD_nuZzmZtatIEAnycc3K-Awi9JficCIU_FoyZXHEpBcWYYIxFnZg-Q8eHi-cP9kfopJRtRSis5Et0RIhUcs3FMbq5SnHXQzYFGntrwtBkMHYMaWjOrjY_PjQ-5Wa8hcbBCEs9-ebaxD4NEKNpKsPkMfhgg4lxV8_JTtGM4KpesL9haHow4yv0wptY4PV-PUW_vnz-ufm2uvz-9WLz6XJlORZ0pVjnLbGUSUfalpK2I150XALm1DuhmCeYdUx0HntnuWJ1rIlpCWmJYpiwU3Sx6Lpktvouh97knU4m6PtCyjd6tmsjaOKEpU5xaC3n1EJn25pKB8Jgs3bGV63zRavYADHpbZryUM3r6zlY_V_6lfB-Idzl9GeCMuo-FDvHNECaim4plYzJGfjuEfCgTVRVrU3yCmoXkM2plAz-0A7Bev4FT_h4s5efuh7cP97-2dlfk5Go3A</recordid><startdate>20011001</startdate><enddate>20011001</enddate><creator>dos Santos, L R</creator><creator>do Nascimento, V P</creator><creator>de Oliveira, S D</creator><creator>Flores, M L</creator><creator>Pontes, A P</creator><creator>Ribeiro, A R</creator><creator>Salle, C T</creator><creator>Lopes, R F</creator><general>Instituto de Medicina Tropical de Sao Paulo</general><general>Instituto de Medicina Tropical</general><general>Universidade de São Paulo (USP)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>CLZPN</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope><scope>7X8</scope><scope>GPN</scope><scope>DOA</scope></search><sort><creationdate>20011001</creationdate><title>Polymerase chain reaction (PCR) for the detection of Salmonella in artificially inoculated chicken meat</title><author>dos Santos, L R ; do Nascimento, V P ; de Oliveira, S D ; Flores, M L ; Pontes, A P ; Ribeiro, A R ; Salle, C T ; Lopes, R F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4052-93bfc1c236d177217b1f5b46e042fd593f103b35bf0fdc49349381a7117193013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Chicken meat</topic><topic>Chickens - 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Academic</collection><collection>SciELO</collection><collection>Directory of Open Access Journals</collection><jtitle>Revista do Instituto de Medicina Tropical de São Paulo</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>dos Santos, L R</au><au>do Nascimento, V P</au><au>de Oliveira, S D</au><au>Flores, M L</au><au>Pontes, A P</au><au>Ribeiro, A R</au><au>Salle, C T</au><au>Lopes, R F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polymerase chain reaction (PCR) for the detection of Salmonella in artificially inoculated chicken meat</atitle><jtitle>Revista do Instituto de Medicina Tropical de São Paulo</jtitle><addtitle>Rev Inst Med Trop Sao Paulo</addtitle><date>2001-10-01</date><risdate>2001</risdate><volume>43</volume><issue>5</issue><spage>247</spage><epage>250</epage><pages>247-250</pages><issn>0036-4665</issn><issn>1678-9946</issn><eissn>0036-4665</eissn><eissn>1678-9946</eissn><coden>RMTSAE</coden><abstract>The aim of this study was to develop a polymerase chain reaction (PCR) protocol for the detection of Salmonella in artificially contaminated chicken meat. Tests were performed with different dilutions of Salmonella Typhimurium or Salmonella Enteritidis cells (10(-7), 10(-8) or 10(-9) CFU/mL) inoculated in chicken meat samples, in order to establish the limits of detection, incubation times (0, 6, 8 and 24 hours of pre-enrichment in PBW 1%) and three DNA extraction protocols (phenol-chloroform, thermal treatment and thermal treatment and Sephaglass). The assay was able to detect until 10(-9) CFU/mL of initial dilution of Salmonella cells inoculated in chicken meat, which allows detection of Salmonella within 48 hours, including 24 hours of pre-enrichment and using the phenol-chloroform DNA extraction protocol. As the results are obtained in a shorter time period than that of microbiological culture, this procedure will be useful in the methodology for detection of Salmonella in chicken.</abstract><cop>Brazil</cop><pub>Instituto de Medicina Tropical de Sao Paulo</pub><pmid>11696845</pmid><doi>10.1590/s0036-46652001000500002</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Revista do Instituto de Medicina Tropical de São Paulo, 2001-10, Vol.43 (5), p.247-250 |
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| language | eng |
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| source | Publicly Available Content Database; SciELO |
| subjects | Animals Chicken meat Chickens - microbiology DNA, Bacterial - analysis DNA, Bacterial - isolation & purification PCR Polymerase Chain Reaction Poultry Products - microbiology Salmonella Salmonella - isolation & purification Time Factors TROPICAL MEDICINE |
| title | Polymerase chain reaction (PCR) for the detection of Salmonella in artificially inoculated chicken meat |
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