The effect of Paclitaxel alone and in combination with Cycloheximide on the frequency of premature centromere division in vitro

Premature centromere division (PCD) can be viewed as a manifestation of chromosome instability. In order to evaluate the ability of Paclitaxel (Ptx) and Cycloheximide (Cy) to induce PCD we used a cytokinesis block micronucleus assay (CBMN), fluorescent in situ hybridization (FISH), and the chromosom...

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Bibliographic Details
Published in:Archives of biological sciences 2010, Vol.62 (1), p.63-74
Main Authors: Bajic, V., Galenika a.d., Belgrade (Serbia). Institute of Biomedical Research, Stanimirovic, Z., University of Belgrade, Belgrade (Serbia). Faculty of Veterinary Medicine, Stevanovic, J., University of Belgrade, Belgrade (Serbia). Faculty of Veterinary Medicine, Milicevic, Z., Vinca Institute of Nuclear Sciences, Belgrade (Serbia). Department for Molecular Biology and Endocrinology, Zivkovic, L., University of Belgrade, Belgrade (Serbia). Faculty of Pharmacy, Spremo-Potparevic, B., University of Belgrade, Belgrade (Serbia). Faculty of Pharmacy
Format: Article
Language:English
Subjects:
CARCINOGENOS
CARCINOGENS
CELL DIVISION
CHROMOSOME
CHROMOSOMES
CROMOSOMAS
cycloheximide
cytokinesis block micronucleus assay(CBMN)
DIVISION CELLULAIRE
DIVISION CELULAR
EXPERIMENTACION IN VITRO
EXPERIMENTATION IN VITRO
fluorescent in situ hybridization (FISH)
GENERO HUMANO
GENRE HUMAIN
http://www.fao.org/aos/agrovoc#c_1313
http://www.fao.org/aos/agrovoc#c_1413
http://www.fao.org/aos/agrovoc#c_1598
http://www.fao.org/aos/agrovoc#c_16131
http://www.fao.org/aos/agrovoc#c_4484
http://www.fao.org/aos/agrovoc#c_4586
IN VITRO EXPERIMENTATION
LINFOCITOS
LYMPHOCYTE
LYMPHOCYTES
MANKIND
Paclitaxel (Ptx)
premature centromere division (PCD)
SUBSTANCE CANCERIGENE
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Summary:Premature centromere division (PCD) can be viewed as a manifestation of chromosome instability. In order to evaluate the ability of Paclitaxel (Ptx) and Cycloheximide (Cy) to induce PCD we used a cytokinesis block micronucleus assay (CBMN), fluorescent in situ hybridization (FISH), and the chromosome aberration (CA) assay in human peripheral blood lymphocytes. Results showed that Ptx can induce PCD alone or in combination with Cy. These findings call us to pay more attention to PCD as a parameter of genotoxicity in the pre-clinical research of mono- and/or combinational therapies for cancer treatment.
ISSN:0354-4664
1821-4339
DOI:10.2298/ABS1001063B