Metabolic Responses of the Microalga Neochloris oleoabundans to Extracellular Self- and Nonself-DNA

Stressed organisms identify intracellular molecules released from damaged cells due to trauma or pathogen infection as components of the innate immune response. These molecules called DAMPs (Damage-Associated Molecular Patterns) are extracellular ATP, sugars, and extracellular DNA, among others. Ani...

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Published in:International journal of molecular sciences 2023-09, Vol.24 (18), p.14172
Main Authors: Zárate-López, Mónica A., Quintana-Rodríguez, Elizabeth, Orona-Tamayo, Domancar, Aguilar-Hernández, Víctor, Araujo-León, Jesús A., Brito-Argáez, Ligia, Molina-Torres, Jorge, Hernández-Flores, José Luis, Loyola-Vargas, Víctor M., Lozoya-Pérez, Nancy E., Lozoya-Gloria, Edmundo
Format: Article
Language:English
Subjects:
Adenosine triphosphate
Algae
Biosynthesis
Carbohydrates
Deoxyribonucleic acid
DNA
Enzymes
Kinases
Lipids
MeJA
Neochloris oleoabundans
nonself-exDNA
peroxidase
phytohormones
Polyphenols
Reactive oxygen species
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Summary:Stressed organisms identify intracellular molecules released from damaged cells due to trauma or pathogen infection as components of the innate immune response. These molecules called DAMPs (Damage-Associated Molecular Patterns) are extracellular ATP, sugars, and extracellular DNA, among others. Animals and plants can recognize their own DNA applied externally (self-exDNA) as a DAMP with a high degree of specificity. However, little is known about the microalgae responses to damage when exposed to DAMPs and specifically to self-exDNAs. Here we compared the response of the oilseed microalgae Neochloris oleoabundans to self-exDNA, with the stress responses elicited by nonself-exDNA, methyl jasmonate (MeJA) and sodium bicarbonate (NaHCO3). We analyzed the peroxidase enzyme activity related to the production of reactive oxygen species (ROS), as well as the production of polyphenols, lipids, triacylglycerols, and phytohormones. After 5 min of addition, self-exDNA induced peroxidase enzyme activity higher than the other elicitors. Polyphenols and lipids were increased by self-exDNA at 48 and 24 h, respectively. Triacylglycerols were increased with all elicitors from addition and up to 48 h, except with nonself-exDNA. Regarding phytohormones, self-exDNA and MeJA increased gibberellic acid, isopentenyladenine, and benzylaminopurine at 24 h. Results show that Neochloris oleoabundans have self-exDNA specific responses.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms241814172