Single Microfluidic Electrochemical Sensor System for Simultaneous Multi-Pulmonary Hypertension Biomarker Analyses

Miniaturized microfluidic biosensors have recently been advanced for portable point-of-care diagnostics by integrating lab-on-a-chip technology and electrochemical analysis. However, the design of a small, integrated, and reliable biosensor for multiple and simultaneous electrochemical analyses in a...

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Bibliographic Details
Published in:Scientific reports 2017-08, Vol.7 (1), p.7545-8, Article 7545
Main Authors: Lee, GeonHui, Lee, JuKyung, Kim, JeongHoon, Choi, Hak Soo, Kim, Jonghan, Lee, SangHoon, Lee, HeaYeon
Format: Article
Language:English
Subjects:
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Adiponectin
Biomarkers
Biomarkers - analysis
Biosensing Techniques - methods
Biosensors
Electrochemical Techniques - methods
Electrochemistry
Fibrinogen
Humanities and Social Sciences
Humans
Hypertension
Hypertension, Pulmonary - diagnosis
Hypertension, Pulmonary - metabolism
Lab-on-a-chip
Lung diseases
Microfluidic Analytical Techniques - methods
Microfluidics
Miniaturization - instrumentation
Miniaturization - methods
multidisciplinary
Point-of-Care Systems
Pulmonary hypertension
Reagents
Reproducibility of Results
Science
Science (multidisciplinary)
Sensitivity and Specificity
Sensors
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Summary:Miniaturized microfluidic biosensors have recently been advanced for portable point-of-care diagnostics by integrating lab-on-a-chip technology and electrochemical analysis. However, the design of a small, integrated, and reliable biosensor for multiple and simultaneous electrochemical analyses in a single device remains a challenge. Here, we present a simultaneous microfluidic electrochemical biosensing system to detect multiple biomarkers of pulmonary hypertension diseases in a single device. The miniaturized biosensor, which is composed of five chambers, is precisely and individually controlled using in-house-built pneumatic microvalves to manipulate the flow pathway. Each chamber is connected to an electrochemical sensor designed to detect four different biomarkers plus a reference control. Our design allows for loading of multiple reagents for simultaneous analyses. On the basis of the developed microfluidic electrochemical sensor system, we successfully detected four well-defined pulmonary hypertension-associated biomarkers, namely, fibrinogen, adiponectin, low-density lipoprotein, and 8-isoprostane. This novel approach offers a new platform for a rapid, miniaturized, and sensitive diagnostic sensor in a single device for various human diseases.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-06144-9