Rational strain improvement for surfactin production: enhancing the yield and generating novel structures

Surfactin, one of the most powerful microbial surfactants, is a lipopeptide-type biosurfactant which combines interesting physicochemical properties and biological activities. However, the high cost caused by its low productivity largely limits the commercial application of surfactin. Hence, many en...

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Bibliographic Details
Published in:Microbial cell factories 2019-02, Vol.18 (1), p.42-42, Article 42
Main Authors: Hu, Fangxiang, Liu, Yuyue, Li, Shuang
Format: Article
Language:English
Subjects:
Amino acids
Antibiotics
Bioengineering
Biological properties
Biosynthesis
Branched chain fatty acids
Chemical properties
Combinatorial analysis
Efflux
Enhanced oil recovery
Enzymes
Fatty acids
Genes
Genetic engineering
Genetic research
Genetically modified organisms
Genomics
Ligases
Microorganisms
Novels
NRPS
Peptides
Physicochemical properties
Pollutants
Production management
Proteins
Review
Structure
Surface active agents
Surfactants
Surfactin
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Summary:Surfactin, one of the most powerful microbial surfactants, is a lipopeptide-type biosurfactant which combines interesting physicochemical properties and biological activities. However, the high cost caused by its low productivity largely limits the commercial application of surfactin. Hence, many engineered bacterium have also been used to enhance surfactin biosynthesis. This review briefly summarizes the mechanism of surfactin biosynthesis, highlighting the synthesis pathway of N-terminally attached fatty acids, and outlines the main genetic engineering strategies for improving the yield and generating novel structures of surfactin, including promoter engineering, enhancing efflux systems, modifying the transcriptional regulatory genes of surfactin synthase (srfA), genomics and transcriptomics analysis, non ribosomal peptide synthetase (NRPS) domain and combinatorial biosynthesis. Finally, we discuss the future prospects of the research on surfactin.
ISSN:1475-2859
1475-2859
DOI:10.1186/s12934-019-1089-x