Effectiveness of the production of tissue-engineered living bone graft: a comparative study using perfusion and rotating bioreactor systems

Bioreactor systems are very precious tools to generate living bone grafts in vitro . The aim of this study was to compare the effectiveness of rotating and perfusion bioreactor in the production of a living bone construct. Human bone marrow-derived mesenchymal stem cells (BMDSCs) were seeded on the...

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Bibliographic Details
Published in:Scientific reports 2023-08, Vol.13 (1), p.13737-13737, Article 13737
Main Authors: Kazimierczak, Paulina, Kalisz, Grzegorz, Sroka-Bartnicka, Anna, Przekora, Agata
Format: Article
Language:English
Subjects:
631/532/2074
631/532/2118
631/61/54/2295
631/61/54/990
631/61/54/991
639/301/54/994
Bioreactors
Bone marrow
Cell culture
Cell number
Cell proliferation
Comparative studies
Cultivation
Extracellular matrix
Humanities and Social Sciences
Hydroxyapatite
Mesenchymal stem cells
Mineralization
multidisciplinary
Osteopontin
Perfusion
Raman spectroscopy
Science
Science (multidisciplinary)
Stem cells
Tissue engineering
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Summary:Bioreactor systems are very precious tools to generate living bone grafts in vitro . The aim of this study was to compare the effectiveness of rotating and perfusion bioreactor in the production of a living bone construct. Human bone marrow-derived mesenchymal stem cells (BMDSCs) were seeded on the surfaces of hydroxyapatite-based scaffolds and cultured for 21 days in three different conditions: (1) static 3D culture, (2) 3D culture in a perfusion bioreactor, and (3) dynamic 3D culture in a rotating bioreactor. Quantitative evaluation of cell number showed that cultivation in the perfusion bioreactor significantly reduced cell proliferation compared to the rotating bioreactor and static culture. Osteogenic differentiation test demonstrated that BMDSCs cultured in the rotating bioreactor produced significantly greater amount of osteopontin compared to the cells cultured in the perfusion bioreactor. Moreover, Raman spectroscopy showed that cultivation of BMDSCs in the rotating bioreactor enhanced extracellular matrix (ECM) mineralization that was characterized by B-type carbonated substitution of hydroxyapatite (associated with PO 4 3− groups) and higher mineral-to-matrix ratio compared to the ECM of cells cultured in the perfusion system. Thus, it was concluded that the rotating bioreactor was much more effective than the perfusion one in the generation of bone tissue construct in vitro.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-41003-w