Evidence for a compartmentation of brain microsomal diacylglycerol

Phosphatidylcholine synthesis from CDP-[methyl-14C]choline and membrane-bound diacyl-[U-14C]-sn-glycerol, formed through the glycerol phosphate pathway, has been examined in vitro in rat brain microsomes. When labeled diacylglycerol was incubated in the presence of unlabeled CDP-choline, the rate of...

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Published in:Journal of lipid research 1982-09, Vol.23 (7), p.955-961
Main Authors: Binaglia, L, Roberti, R, Vecchini, A, Porcellati, G
Format: Article
Language:English
Subjects:
Animals
brain
Brain - metabolism
Carbon Radioisotopes
Cytidine Diphosphate Choline - metabolism
Cytidine Monophosphate - metabolism
diacylglycerol
Diglycerides - metabolism
Glycerides - metabolism
Kinetics
microsomes
Microsomes - metabolism
Phosphatidylcholines - biosynthesis
Phospholipases
Rats
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description Phosphatidylcholine synthesis from CDP-[methyl-14C]choline and membrane-bound diacyl-[U-14C]-sn-glycerol, formed through the glycerol phosphate pathway, has been examined in vitro in rat brain microsomes. When labeled diacylglycerol was incubated in the presence of unlabeled CDP-choline, the rate of phospholipid labeling looked very different from that measured in incubations of unlabeled diacylglycerol with CDP-[methyl-14C]choline. Evidence is given that diacylglycerol formed through the glycerol phosphate pathway belongs to a metabolic pool separate from the bulk membrane diacylglycerol.
doi_str_mv 10.1016/S0022-2275(20)38067-6
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subjects Animals
brain
Brain - metabolism
Carbon Radioisotopes
Cytidine Diphosphate Choline - metabolism
Cytidine Monophosphate - metabolism
diacylglycerol
Diglycerides - metabolism
Glycerides - metabolism
Kinetics
microsomes
Microsomes - metabolism
Phosphatidylcholines - biosynthesis
Phospholipases
Rats
title Evidence for a compartmentation of brain microsomal diacylglycerol
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