Glycoproteins Involved in Sea Urchin Temporary Adhesion

Biomedical adhesives, despite having been used increasingly in recent years, still face a major technological challenge: strong adhesion in wet environments. In this context, biological adhesives secreted by marine invertebrates have appealing characteristics to incorporate into new underwater biomi...

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Bibliographic Details
Published in:Marine drugs 2023-02, Vol.21 (3), p.145
Main Authors: Ventura, Inês, Harman, Victoria, Beynon, Robert J, Santos, Romana
Format: Article
Language:English
Subjects:
Adhesion
Adhesive strength
Adhesives
Adhesives - chemistry
Adhesives - metabolism
Analytical methods
Animals
bioadhesive
Biodegradability
Biodegradation
biomimetic adhesive
Biomimetics
Chemical properties
Cohesion
Glycoproteins
Glycoproteins - metabolism
Histology
Identification and classification
Invertebrates
Lectins
Lectins - metabolism
Marine invertebrates
Mass Spectrometry
Mass spectroscopy
Materials research
Molecular weight
N-Acetylglucosamine
Nectin
Paracentrotus - metabolism
Physiological aspects
Proteins
sea urchin
Sea urchins
Sugar
Transcriptomics
tube feet
Underwater
Water resistance
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Summary:Biomedical adhesives, despite having been used increasingly in recent years, still face a major technological challenge: strong adhesion in wet environments. In this context, biological adhesives secreted by marine invertebrates have appealing characteristics to incorporate into new underwater biomimetic adhesives: water resistance, nontoxicity and biodegradability. Little is still known about temporary adhesion. Recently, a transcriptomic differential analysis of sea urchin tube feet pinpointed 16 adhesive/cohesive protein candidates. In addition, it has been demonstrated that the adhesive secreted by this species is composed of high molecular weight proteins associated with N-Acetylglucosamine in a specific chitobiose arrangement. As a follow-up, we aimed to investigate which of these adhesive/cohesive protein candidates were glycosylated through lectin pulldowns, protein identification by mass spectroscopy and in silico characterization. We demonstrate that at least five of the previously identified protein adhesive/cohesive candidates are glycoproteins. We also report the involvement of a third Nectin variant, the first adhesion-related protein to be identified in . By providing a deeper characterization of these adhesive/cohesive glycoproteins, this work advances our understanding of the key features that should be replicated in future sea urchin-inspired bioadhesives.
ISSN:1660-3397
1660-3397
DOI:10.3390/md21030145