The neurotoxic effects of hydrogen peroxide and copper in Retzius nerve cells of the leech Haemopis sanguisuga

• Published in: Biology open 2016-04, Vol.5 (4), p.381-388
• Main Authors: Jovanovic, Zorica D, Stanojevic, Marija B, Nedeljkov, Vladimir B
• Format: Article
• Language: English
• Subjects: Antioxidants; Channels; Copper; Dimethyl sulfoxide; Electrical properties; Electrophysiological recording; Haemopis; Hydrogen peroxide; Hydroxyl radicals; Leech; Leeches; Mannitol; Membrane potential; Membranes; Nerves; Neurons; Neurotoxicity; Oxidants; Oxidation; Oxidative stress; Oxidizing agents; Potassium; Potassium channels; Potassium current; Protein transport; Reactive oxygen species
• ISSN: 2046-6390; 2046-6390
• DOI: 10.1242/bio.014936

Oxidative stress and the generation of reactive oxygen species (ROS) play an important role in cellular damage. Electrophysiological analyses have shown that membrane transport proteins are susceptible to ROS. In the present study, oxidative stress was induced in Retzius nerve cells of the leechHaemopis sanguisugaby bath application of 1 mM of hydrogen peroxide (H2O2) and 0.02 mM of copper (Cu) for 20 min. The H2O2/Cu(II) produced considerable changes in the electrical properties of the Retzius nerve cells. Intracellular recording of the resting membrane potential revealed that the neuronal membrane was depolarized in the presence of H2O2/Cu(II). We found that the amplitude of action potentials decreased, while the duration augmented in a progressive way along the drug exposure time. The combined application of H2O2and Cu(II) caused an initial excitation followed by depression of the spontaneous electrical activity. Voltage-clamp recordings revealed a second effect of the oxidant, a powerful inhibition of the outward potassium channels responsible for the repolarization of action potentials. The neurotoxic effect of H2O2/Cu(II) on the spontaneous spike electrogenesis and outward K(+)current of Retzius nerve cells was reduced in the presence of hydroxyl radical scavengers, dimethylthiourea and dimethyl sulfoxide, but not mannitol. This study provides evidence for the oxidative modification of outward potassium channels in Retzius nerve cells. The oxidative mechanism of the H2O2/Cu(II) system action on the electrical properties of Retzius neurons proposed in this study might have a wider significance, referring not only to leeches but also to mammalian neurons.