Occurrence of Antibiotics and Antibiotic-Resistant Bacteria in Landfill Sites in Kumasi, Ghana

The incidence of antimicrobial resistance among microbial communities is a major threat to global health care and security. Landfills, which are reservoirs for many pharmaceuticals, provide a conducive habitat for antimicrobial-resistant microbes and resistant gene transfer and are therefore a major...

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Bibliographic Details
Published in:Journal of chemistry 2019, Vol.2019 (2019), p.1-10
Main Authors: Woode, Eric, Boamah, Vivian Etsiapa, Lartey, Jemima Asi, Nsiah, Sarah Twumwah, Ahor, Hubert Senanu, Darko, Godfred, Ekuadzi, Edmund, Borquaye, Lawrence Sheringham, Mutala, Abdul-Hakim
Format: Article
Language:English
Subjects:
Abandoned waste sites
Amoxicillin
Antibiotics
Antiinfectives and antibacterials
Antimicrobial agents
Apoptosis
Bacteria
Bacterial leaching
Chemistry
Drug resistance
Environmental science
Genes
Landfill
Landfills
Leachates
Listeria
Metabolites
Microorganisms
Municipal solid waste
Penicillin
Pharmaceuticals
Public health
Soil resistance
Solid phases
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Summary:The incidence of antimicrobial resistance among microbial communities is a major threat to global health care and security. Landfills, which are reservoirs for many pharmaceuticals, provide a conducive habitat for antimicrobial-resistant microbes and resistant gene transfer and are therefore a major contributor to the phenomenon of antimicrobial resistance. Hence, this study determined the levels of three widely used antibiotics, metronidazole, penicillin, and amoxicillin, and the occurrence of antimicrobial resistance amongst microbes in soil and leachate samples from active and abandoned landfill sites in Kumasi, Ghana. Soil samples were collected from one active and four abandoned landfills, while leachate specimen was collected only from the active landfill. Sonication and solid-phase extraction (SPE) were used for sample preparation, followed by analysis via an HPLC-PDA method. Isolation and characterization of bacteria were done using standard bacteriological techniques. Antibiotic susceptibility testing was determined following the European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines. Antibiotics were detected at very high concentrations in the specimen collected from both active and abandoned landfill sites. For leachate samples obtained from Dompoase, penicillin was present at the highest concentration (67.42 ± 5.35 μg/mL, p
ISSN:2090-9063
2090-9071
DOI:10.1155/2019/6934507