Evaluation of Perfluorohexyloctane/Polydimethylsiloxane for Pancreas Preservation for Clinical Islet Isolation and Transplantation

This study aimed to evaluate a 50:50 mix of perfluorohexyloctane/polydimethylsiloxane 5 (F6H8S5) preservation of pancreases in a clinical setting compared with standard solutions for 1) cold ischemia time (CIT) 20 h. Procured clinical-grade pancreases were shipped in either F6H8S5 or in standard pre...

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Bibliographic Details
Published in:Cell transplantation 2016-01, Vol.25 (12), p.2269-2276
Main Authors: Ståhle, Magnus, Foss, Aksel, Gustafsson, Bengt, Lempinen, Marko, Lundgren, Torbjörn, Rafael, Ehab, Tufveson, Gunnar, Theisinger, Bastian, Brandhorst, Daniel, Korsgren, Olle, Friberg, Andrew
Format: Article
Language:English
Subjects:
Adult
Aged
Cancer and Oncology
Cancer och onkologi
Clinical islet transplantation
Clinical Medicine
Dimethylpolysiloxanes - chemistry
Duodenum
Fluorocarbons - chemistry
Humans
Hypoxia
Hypoxia - metabolism
Ischemia
Islet cells
Islet isolation
Islets of Langerhans Transplantation - methods
Kirurgi
Klinisk medicin
Medical and Health Sciences
Medicin och hälsovetenskap
Middle Aged
Organ Preservation Solutions - chemistry
Oxygenation
Pancreas - metabolism
Pancreas preservation
Pancreas transplantation
Pancreatic islet transplantation
Perfluorohydrocarbons
Polydimethylsiloxane
Preservation
Surgery
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Summary:This study aimed to evaluate a 50:50 mix of perfluorohexyloctane/polydimethylsiloxane 5 (F6H8S5) preservation of pancreases in a clinical setting compared with standard solutions for 1) cold ischemia time (CIT) 20 h. Procured clinical-grade pancreases were shipped in either F6H8S5 or in standard preservation solutions, that is, University of Wisconsin (UW) or Custodiol. F6H5S5 was preoxygenated for at least 15 min. Included clinical-grade pancreases were procured in UW or Custodiol. Upon arrival at the islet isolation laboratory, the duodenum was removed followed by rough trimming while F6H8S5 was oxygenated for 15-20 min. Trimmed pancreases were immersed into oxygenated F6H8S5 and stored at 4°C overnight followed by subsequent islet isolation. Pancreas preservation using F6H8S5 proved as effective as UW and Custadiol when used within CIT up to 10 h, in terms of both isolation outcome and islet functionality. Preservation in F6H8S5 of pancreases with extended CIT gave results similar to controls with CIT
ISSN:0963-6897
1555-3892
1555-3892
DOI:10.3727/096368916X691709