Drought specifically downregulates mineral nutrition: Plant ionomic content and associated gene expression

• Published in: Plant direct 2022-08, Vol.6 (8), p.e402-n/a
• Main Authors: D'Oria, Aurélien, Courbet, Galatéa, Billiot, Bastien, Jing, Lun, Pluchon, Sylvain, Arkoun, Mustapha, Maillard, Anne, Roux, Christine Paysant‐Le, Trouverie, Jacques, Etienne, Philippe, Diquélou, Sylvain, Ourry, Alain
• Format: Article
• Language: English
• Subjects: Abiotic stress; Climate change; Drought; Environmental Sciences; Environmental stress; Gene expression; Homeostasis; ionome; iron; known ionomic genes; Manganese; molybdenum; Morphology; nutrient; Nutrients; Ontology; Original Research; Physiology; Plant growth; Rain; Soils; transport associated genes; water deficit; zinc
• ISSN: 2475-4455; 2475-4455
• DOI: 10.1002/pld3.402

One of the main limiting factors of plant yield is drought, and while the physiological responses to this environmental stress have been broadly described, research addressing its impact on mineral nutrition is scarce. Brassica napus and Triticum aestivum were subjected to moderate or severe water deficit, and their responses to drought were assessed by functional ionomic analysis, and derived calculation of the net uptake of 20 nutrients. While the uptake of most mineral nutrients decreased, Fe, Zn, Mn, and Mo uptake were impacted earlier and at a larger scale than most physiological parameters assessed (growth, ABA concentration, gas exchanges and photosynthetic activity). Additionally, in B. napus, the patterns of 183 differentially expressed genes in leaves related to the ionome (known ionomic genes, KIGs) or assumed to be involved in transport of a given nutrient were analyzed. This revealed three patterns of gene expression under drought consisting of up (transport of Cl and Co), down (transport of N, P, B, Mo, and Ni), or mixed levels (transport of S, Mg, K, Zn, Fe, Cu, or Mn) of regulation. The three patterns of gene regulations are discussed in relation to specific gene functions, changes of leaf ionomic composition and with consideration of the crosstalks that have been established between elements. It is suggested that the observed reduction in Fe uptake occurred via a specific response to drought, leading indirectly to reduced uptake of Zn and Mn, and these may be taken up by common transporters encoded by genes that were downregulated. Highlight In wheat and rapeseed, drought downregulates the uptake of Fe, Mo, Zn, and Mn before reduction of growth, and this was associated with differential expressions of transport‐related genes.