Asymptomatic Plasmodium falciparum malaria prevalence among adolescents and adults in Malawi, 2015–2016

• Published in: Scientific reports 2020-10, Vol.10 (1), p.18740-11, Article 18740
• Main Authors: Topazian, Hillary M., Gumbo, Austin, Puerto-Meredith, Sydney, Njiko, Ruth, Mwanza, Alexis, Kayange, Michael, Mwalilino, David, Mvula, Bernard, Tegha, Gerald, Mvalo, Tisungane, Edwards, Jessie K., Emch, Michael, Pettifor, Audrey, Smith, Jennifer S., Hoffman, Irving, Meshnick, Steven R., Juliano, Jonathan J.
• Format: Article
• Language: English
• Subjects: 692/308/174; 692/699/255/1629; Adolescent; Adult; Asymptomatic; Asymptomatic infection; Children; Education; Erythrocytes; Female; Humanities and Social Sciences; Humans; Infections; Insecticide-Treated Bednets; Insecticides; Malaria; Malaria, Falciparum - epidemiology; Malawi - epidemiology; Male; Middle Aged; Morbidity; multidisciplinary; Parasites; Plasmodium falciparum; Plasmodium falciparum - pathogenicity; Polymerase chain reaction; Prevalence; Science; Science (multidisciplinary); Temperature; Vector-borne diseases; Young Adult
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-75261-9

Malaria remains a significant cause of morbidity and mortality in Malawi, with an estimated 18–19% prevalence of Plasmodium falciparum in children 2–10 years in 2015–2016. While children report the highest rates of clinical disease, adults are thought to be an important reservoir to sustained transmission due to persistent asymptomatic infection. The 2015–2016 Malawi Demographic and Health Survey was a nationally representative household survey which collected dried blood spots from 15,125 asymptomatic individuals ages 15–54 between October 2015 and February 2016. We performed quantitative polymerase chain reaction on 7,393 samples, detecting an overall P. falciparum prevalence of 31.1% (SE = 1.1). Most infections (55.6%) had parasitemias ≤ 10 parasites/µL. While 66.2% of individuals lived in a household that owned a bed net, only 36.6% reported sleeping under a long-lasting insecticide-treated net (LLIN) the previous night. Protective factors included urbanicity, greater wealth, higher education, and lower environmental temperatures. Living in a household with a bed net (prevalence difference 0.02, 95% CI − 0.02 to 0.05) and sleeping under an LLIN (0.01; − 0.02 to 0.04) were not protective against infection. Our findings demonstrate a higher parasite prevalence in adults than published estimates among children. Understanding the prevalence and distribution of asymptomatic infection is essential for targeted interventions.