Intra-tumoral YAP and TAZ heterogeneity drives collective NSCLC invasion that is targeted by SUMOylation inhibitor TAK-981

Non-small cell lung cancer (NSCLC) collective invasion is supported by cooperativity of proliferative (follower) and invasive (leader) cells. H1299-isolated follower cells exhibit higher Yes-associated protein (YAP) expression, while leader cells were found to express elevated transcriptional coacti...

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Published in:iScience 2024-11, Vol.27 (11), p.111133, Article 111133
Main Authors: Sharma, Richa, Sharma, Shagun, Shriwas, Pratik, Mehta, Labdhi, Vu, An H., Mouw, Janna K., Koo, Junghui, Huang, Chunzi, Matsuk, Veronika Y., Tucker-Burden, Carol, Joseph, Gregory, Behera, Madhusmita, Sun, Shi-Yong, Roy, Melissa A., Gilbert-Ross, Melissa, Leal, Ticiana, Marcus, Adam I., Shanmugam, Mala
Format: Article
Language:English
Subjects:
Cancer
Cell biology
Microenvironment
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Summary:Non-small cell lung cancer (NSCLC) collective invasion is supported by cooperativity of proliferative (follower) and invasive (leader) cells. H1299-isolated follower cells exhibit higher Yes-associated protein (YAP) expression, while leader cells were found to express elevated transcriptional coactivator with PDZ-binding motif (TAZ/WWTR1) expression. Suppressing TAZ (not YAP) in leader cells reduced invasion. TAZ-regulated leader cell invasion is associated with activation of the EGFR-PI3K-AKT axis. NSCLC patient samples also demonstrated heterogeneity in YAP and TAZ expression. YAP and TAZ regulate proliferation of follower and leader cells. Our results highlight the need to inhibit both YAP and TAZ to effectively target their regulation of collective invasion. We identify that the SUMOylation inhibitor TAK-981 reduces YAP and TAZ expression, decreasing tumor burden and metastasis in a murine NSCLC model. Our study reveals an intra-tumoral division of labor, driven by differential YAP and TAZ expression, which can be effectively targeted with TAK-981 for NSCLC therapy. [Display omitted] •Leader and follower cells exhibit differential YAP and TAZ dependencies•TAZ-induced invasion in leader cells involves activation of EGFR-PI3K/AKT signaling•Reduced βTrCP in leader cells elevates TAZ expression•TAK-981 suppresses YAP, TAZ, and metastasis in preclinical NSCLC Microenvironment; Cell biology; Cancer
ISSN:2589-0042
2589-0042
DOI:10.1016/j.isci.2024.111133