Correlation between Anti-Toxoplasma gondii IgG Antibodies in Serum and Colostrum of Naturally Infected Sheep and Passive Immunization in Lambs

• Published in: Microbiology research 2024-09, Vol.15 (3), p.1806-1813
• Main Authors: Alvares, Felipe Boniedj Ventura, Lima, Brendo Andrade, Lima, Ana Maria Santos, Batista, Samira Pereira, Aguiar, Antônia Aniellen Raianne Moisés, Ferreira, Larissa Claudino, Silva, Welitânia Inácia, Feitosa, Thais Ferreira, Vilela, Vinícius Longo Ribeiro
• Format: Article
• Language: English
• Subjects: Abortion; Agreements; Animals; Antibodies; antibody transfer; Births; Colostrum; colostrum analysis; Cysts; Economic impact; Farms; Immunity (passive); Immunization; Immunization (passive); Immunoglobulin G; Kappa coefficient; Lamb; Livestock farming; Parasites; passive immunity; Protozoa; Reproduction; Serology; Sheep; Toxoplasma gondii; Toxoplasmosis
• ISSN: 2036-7481; 2036-7481
• DOI: 10.3390/microbiolres15030120

Toxoplasmosis, caused by Toxoplasma gondii, poses a significant threat to sheep flocks, affecting reproductive performance and meat quality, and leading to economic losses. This study aimed to evaluate the correlation between anti-T. gondii IgG antibodies in the serum and colostrum of naturally infected ewes and to assess passive immunity in newborn lambs. Blood and colostrum samples were collected from 162 ewes and 182 lambs across 20 sheep farms in Paraíba, Brazil. Samples were tested for anti-T. gondii and anti-Neospora caninum IgG using indirect fluorescence antibody tests (IFATs), with titers ≥ 1:64 considered positive. Among the ewes, 45.1% tested positive for anti-T. gondii IgG in serum, with titers ranging from 1:64 to 1:16,384. The colostrum from 94.6% of the ewes also tested positive, although 74% had higher titers in their serum than in their colostrum. Concordance between serum and colostrum was high, with a kappa coefficient of 0.950. Lamb serum showed a perfect agreement with maternal colostrum (kappa = 0.962), demonstrating effective passive transfer of antibodies. This study confirms that colostrum is a reliable matrix for detecting anti-T. gondii antibodies and assessing passive immunity in lambs. The high concordance between serum, colostrum, and lamb titers suggests that IFATs on colostrum can be a practical tool for monitoring maternal antibody transfer, contributing to the better management of T. gondii infections in sheep flocks.