Immobilization of Alcalase on Silica Supports Modified with Carbosilane and PAMAM Dendrimers

Enzyme immobilization is a powerful strategy for enzyme stabilization and recyclability. Materials covered with multipoint molecules are very attractive for this goal, since the number of active moieties to attach the enzyme increases with respect to monofunctional linkers. This work evaluates diffe...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-12, Vol.23 (24), p.16102
Main Authors: Sánchez-Milla, María, Hernández-Corroto, Ester, Sánchez-Nieves, Javier, Gómez, Rafael, Marina, María Luisa, García, María Concepción, de la Mata, F Javier
Format: Article
Language:English
Subjects:
alcalase
Amino acids
Calcium ions
carbosilane dendrimer
Dendrimers
Dendrimers - chemistry
Digestion
enzyme immobilization
Enzymes
Enzymes, Immobilized - chemistry
Immobilization
Morphology
PAMAM dendrimer
Peptides
Recyclability
Silica
Silicon dioxide
Silicon Dioxide - chemistry
Subtilisin
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Summary:Enzyme immobilization is a powerful strategy for enzyme stabilization and recyclability. Materials covered with multipoint molecules are very attractive for this goal, since the number of active moieties to attach the enzyme increases with respect to monofunctional linkers. This work evaluates different dendrimers supported on silica to immobilize a protease enzyme, Alcalase. Five different dendrimers were employed: two carbosilane (CBS) dendrimers of different generations (SiO -G Si-NH and SiO -G Si-NH ), a CBS dendrimer with a polyphenoxo core (SiO -G O -NH ), and two commercial polyamidoamine (PAMAM) dendrimers of different generations (SiO -G PAMAM-NH and SiO -G PAMAM-NH ). The results were compared with a silica support modified with a monofunctional molecule (2-aminoethanethiol). The effect of the dendrimer generation, the immobilization conditions (immobilization time, Alcalase/SiO ratio, and presence of Ca ions), and the digestion conditions (temperature, time, amount of support, and stirring speed) on Alcalase activity has been evaluated. Enzyme immobilization and its activity were highly affected by the kind of dendrimer and its generation, observing the most favorable behavior with SiO -G PAMAM-NH . The enzyme immobilized on this support was used in two consecutive digestions and, unlike CBS supports, it did not retain peptides released in the digestion.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms232416102