The 33.1 kDa Excretory/secretory Protein Produced by Toxocara canis Larvae Serves as a Potential Common Biomarker for Serodiagnosis of Toxocariasis in Paratenic Animals and Human

Toxocariasis is a prevalent zoonosis disease caused by the closely related nematode species and which parasitise Canidae and Felidae respectively. In paratenic hosts, larvae of these worms cause multiple organ damage. However, how these paratenic hosts response to these worms and whether any common...

Full description

Saved in:
Bibliographic Details
Published in:Iranian journal of parasitology 2017, Vol.12 (1), p.69-82
Main Authors: Nguyen, Huu-Hung, Vo, Doan-Trung, Thai, Thi-Tuyet-Trinh, LE, Thi-Thanh-Thao, LE, Thanh-Dong, Hoang, Nghia-Son
Format: Article
Language:English
Subjects:
Animals
Antigens
Biomarkers
Cytokines
Eggs
Excreted/secreted proteins
IgG response
Immunoglobulins
Infections
Molecular weight
Nematodes
Original
Parasitology
Paratenic hosts
Pharmacy
Proteins
Toxocara
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Toxocariasis is a prevalent zoonosis disease caused by the closely related nematode species and which parasitise Canidae and Felidae respectively. In paratenic hosts, larvae of these worms cause multiple organ damage. However, how these paratenic hosts response to these worms and whether any common biomarker can be applied for diagnosis are still unclear. Excreted/secreted (E/S) antigens were prepared by culture of larvae in vitro. Using a western blot (WB) assay the humoral IgG responses, induced by spp. larvae to the worm's E/S antigens in different infected hosts including mice, rabbits and human, were examined. In a mouse model of toxocariasis, intraperitoneal injection of larvae induces inflammatory leukocyte accumulation in the liver and the lungs but not in the brain, although a remarkable number of larvae were detected in this organ. Mice and rabbits responded differently to spp. resulting in distinct heterogenous WB band patterns. Mice and rabbits both responded to a 33.1 kDa E/S constituent that turned out to be the most sensitive protein for serodiagnosis. Sera from human toxocariasis patients showed heterogenous WB band patterns similar to those observed in rabbits and all responded to the 33.1 kDa band. 33.1 kDa E/S protein can be considered as a critical common biomarker for toxocariasis immuno-diagnosis in both paratenic animals and human and its specificity requires further investigation.
ISSN:1735-7020
2008-238X