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The 33.1 kDa Excretory/secretory Protein Produced by Toxocara canis Larvae Serves as a Potential Common Biomarker for Serodiagnosis of Toxocariasis in Paratenic Animals and Human
Toxocariasis is a prevalent zoonosis disease caused by the closely related nematode species and which parasitise Canidae and Felidae respectively. In paratenic hosts, larvae of these worms cause multiple organ damage. However, how these paratenic hosts response to these worms and whether any common...
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| Published in: | Iranian journal of parasitology 2017, Vol.12 (1), p.69-82 |
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| container_title | Iranian journal of parasitology |
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| creator | Nguyen, Huu-Hung Vo, Doan-Trung Thai, Thi-Tuyet-Trinh LE, Thi-Thanh-Thao LE, Thanh-Dong Hoang, Nghia-Son |
| description | Toxocariasis is a prevalent zoonosis disease caused by the closely related nematode species
and
which parasitise Canidae and Felidae respectively. In paratenic hosts, larvae of these worms cause multiple organ damage. However, how these paratenic hosts response to these worms and whether any common biomarker can be applied for diagnosis are still unclear.
Excreted/secreted (E/S) antigens were prepared by culture of
larvae in vitro. Using a western blot (WB) assay the humoral IgG responses, induced by
spp. larvae to the worm's E/S antigens in different infected hosts including mice, rabbits and human, were examined.
In a mouse model of toxocariasis, intraperitoneal injection of
larvae induces inflammatory leukocyte accumulation in the liver and the lungs but not in the brain, although a remarkable number of larvae were detected in this organ. Mice and rabbits responded differently to
spp. resulting in distinct heterogenous WB band patterns. Mice and rabbits both responded to a 33.1 kDa E/S constituent that turned out to be the most sensitive protein for serodiagnosis. Sera from human toxocariasis patients showed heterogenous WB band patterns similar to those observed in rabbits and all responded to the 33.1 kDa band.
33.1 kDa E/S protein can be considered as a critical common biomarker for toxocariasis immuno-diagnosis in both paratenic animals and human and its specificity requires further investigation. |
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and
which parasitise Canidae and Felidae respectively. In paratenic hosts, larvae of these worms cause multiple organ damage. However, how these paratenic hosts response to these worms and whether any common biomarker can be applied for diagnosis are still unclear.
Excreted/secreted (E/S) antigens were prepared by culture of
larvae in vitro. Using a western blot (WB) assay the humoral IgG responses, induced by
spp. larvae to the worm's E/S antigens in different infected hosts including mice, rabbits and human, were examined.
In a mouse model of toxocariasis, intraperitoneal injection of
larvae induces inflammatory leukocyte accumulation in the liver and the lungs but not in the brain, although a remarkable number of larvae were detected in this organ. Mice and rabbits responded differently to
spp. resulting in distinct heterogenous WB band patterns. Mice and rabbits both responded to a 33.1 kDa E/S constituent that turned out to be the most sensitive protein for serodiagnosis. Sera from human toxocariasis patients showed heterogenous WB band patterns similar to those observed in rabbits and all responded to the 33.1 kDa band.
33.1 kDa E/S protein can be considered as a critical common biomarker for toxocariasis immuno-diagnosis in both paratenic animals and human and its specificity requires further investigation.</description><identifier>ISSN: 1735-7020</identifier><identifier>EISSN: 2008-238X</identifier><identifier>PMID: 28761463</identifier><language>eng</language><publisher>Iran: Tehran University of Medical Sciences</publisher><subject>Animals ; Antigens ; Biomarkers ; Cytokines ; Eggs ; Excreted/secreted proteins ; IgG response ; Immunoglobulins ; Infections ; Molecular weight ; Nematodes ; Original ; Parasitology ; Paratenic hosts ; Pharmacy ; Proteins ; Toxocara</subject><ispartof>Iranian journal of parasitology, 2017, Vol.12 (1), p.69-82</ispartof><rights>Copyright Dr Ali Akbari Sari, Director of The Commission for Accreditation & Improvement of Iranian Medical Journals Jan-Mar 2017</rights><rights>2017. This work is published under https://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright© Iranian Society of Parasitology & Tehran University of Medical Sciences 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2840685083/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2840685083?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4010,25731,36989,36990,44566,53766,53768,74869</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28761463$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nguyen, Huu-Hung</creatorcontrib><creatorcontrib>Vo, Doan-Trung</creatorcontrib><creatorcontrib>Thai, Thi-Tuyet-Trinh</creatorcontrib><creatorcontrib>LE, Thi-Thanh-Thao</creatorcontrib><creatorcontrib>LE, Thanh-Dong</creatorcontrib><creatorcontrib>Hoang, Nghia-Son</creatorcontrib><title>The 33.1 kDa Excretory/secretory Protein Produced by Toxocara canis Larvae Serves as a Potential Common Biomarker for Serodiagnosis of Toxocariasis in Paratenic Animals and Human</title><title>Iranian journal of parasitology</title><addtitle>Iran J Parasitol</addtitle><description>Toxocariasis is a prevalent zoonosis disease caused by the closely related nematode species
and
which parasitise Canidae and Felidae respectively. In paratenic hosts, larvae of these worms cause multiple organ damage. However, how these paratenic hosts response to these worms and whether any common biomarker can be applied for diagnosis are still unclear.
Excreted/secreted (E/S) antigens were prepared by culture of
larvae in vitro. Using a western blot (WB) assay the humoral IgG responses, induced by
spp. larvae to the worm's E/S antigens in different infected hosts including mice, rabbits and human, were examined.
In a mouse model of toxocariasis, intraperitoneal injection of
larvae induces inflammatory leukocyte accumulation in the liver and the lungs but not in the brain, although a remarkable number of larvae were detected in this organ. Mice and rabbits responded differently to
spp. resulting in distinct heterogenous WB band patterns. Mice and rabbits both responded to a 33.1 kDa E/S constituent that turned out to be the most sensitive protein for serodiagnosis. Sera from human toxocariasis patients showed heterogenous WB band patterns similar to those observed in rabbits and all responded to the 33.1 kDa band.
33.1 kDa E/S protein can be considered as a critical common biomarker for toxocariasis immuno-diagnosis in both paratenic animals and human and its specificity requires further investigation.</description><subject>Animals</subject><subject>Antigens</subject><subject>Biomarkers</subject><subject>Cytokines</subject><subject>Eggs</subject><subject>Excreted/secreted proteins</subject><subject>IgG response</subject><subject>Immunoglobulins</subject><subject>Infections</subject><subject>Molecular weight</subject><subject>Nematodes</subject><subject>Original</subject><subject>Parasitology</subject><subject>Paratenic hosts</subject><subject>Pharmacy</subject><subject>Proteins</subject><subject>Toxocara</subject><issn>1735-7020</issn><issn>2008-238X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNp9kt9vFCEQxzdGYy-1_4Ih8cWXs_xaYF9M6rW2TS6xiWfiG2FhuHLdhcruXnr_ln-hnL0a64OEZGD4zodhhhfVjGKs5pSp7y-rGZGsnktM8VF1MgwbXAajSgjxujqiSgrCBZtVP1e3gBj7QNDduUEXDzbDmPLudIDDCt3kNEKIe-smCw61O7RKD8mabJA1MQxoafLWAPoKeQsDMmWimxIUx2A6tEh9nyL6FFJv8h1k5FPeS5MLZh3TUOKTfyIGs9_vbyv0QggWncXQm64wo0NXU2_im-qVLw44Odjj6tvni9Xiar78cnm9OFvOHcNsnDMOHNeSOyqEV2BbDqThLThjy8uZoK33hJgWagGNxI2yyntouZXE26Zx7Li6fuS6ZDb6Ppc08k4nE_RvR8prbfIYbAeagPSuAck4A84FNco5SZz1tG5k6UFhfXxk3U9tD86W2mTTPYM-P4nhVq_TVtc1pRKTAnh_AOT0Y4Jh1H0YLHSdiZCmQZOG1qWrkrMiffePdJOmHEupNFUcC1Vj9V8VUZILIjDmRfX277z_JPz0gdgvGOPFlA</recordid><startdate>2017</startdate><enddate>2017</enddate><creator>Nguyen, Huu-Hung</creator><creator>Vo, Doan-Trung</creator><creator>Thai, Thi-Tuyet-Trinh</creator><creator>LE, Thi-Thanh-Thao</creator><creator>LE, Thanh-Dong</creator><creator>Hoang, Nghia-Son</creator><general>Tehran University of Medical Sciences</general><scope>NPM</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>2017</creationdate><title>The 33.1 kDa Excretory/secretory Protein Produced by Toxocara canis Larvae Serves as a Potential Common Biomarker for Serodiagnosis of Toxocariasis in Paratenic Animals and Human</title><author>Nguyen, Huu-Hung ; Vo, Doan-Trung ; Thai, Thi-Tuyet-Trinh ; LE, Thi-Thanh-Thao ; LE, Thanh-Dong ; Hoang, Nghia-Son</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-d303t-34e40574d266f8ecb4e194bedac463362bff11abe56e97098c8ffeb4c71fc99d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Antigens</topic><topic>Biomarkers</topic><topic>Cytokines</topic><topic>Eggs</topic><topic>Excreted/secreted proteins</topic><topic>IgG response</topic><topic>Immunoglobulins</topic><topic>Infections</topic><topic>Molecular weight</topic><topic>Nematodes</topic><topic>Original</topic><topic>Parasitology</topic><topic>Paratenic hosts</topic><topic>Pharmacy</topic><topic>Proteins</topic><topic>Toxocara</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nguyen, Huu-Hung</creatorcontrib><creatorcontrib>Vo, Doan-Trung</creatorcontrib><creatorcontrib>Thai, Thi-Tuyet-Trinh</creatorcontrib><creatorcontrib>LE, Thi-Thanh-Thao</creatorcontrib><creatorcontrib>LE, Thanh-Dong</creatorcontrib><creatorcontrib>Hoang, Nghia-Son</creatorcontrib><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Open Access: DOAJ - Directory of Open Access Journals</collection><jtitle>Iranian journal of parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nguyen, Huu-Hung</au><au>Vo, Doan-Trung</au><au>Thai, Thi-Tuyet-Trinh</au><au>LE, Thi-Thanh-Thao</au><au>LE, Thanh-Dong</au><au>Hoang, Nghia-Son</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The 33.1 kDa Excretory/secretory Protein Produced by Toxocara canis Larvae Serves as a Potential Common Biomarker for Serodiagnosis of Toxocariasis in Paratenic Animals and Human</atitle><jtitle>Iranian journal of parasitology</jtitle><addtitle>Iran J Parasitol</addtitle><date>2017</date><risdate>2017</risdate><volume>12</volume><issue>1</issue><spage>69</spage><epage>82</epage><pages>69-82</pages><issn>1735-7020</issn><eissn>2008-238X</eissn><abstract>Toxocariasis is a prevalent zoonosis disease caused by the closely related nematode species
and
which parasitise Canidae and Felidae respectively. In paratenic hosts, larvae of these worms cause multiple organ damage. However, how these paratenic hosts response to these worms and whether any common biomarker can be applied for diagnosis are still unclear.
Excreted/secreted (E/S) antigens were prepared by culture of
larvae in vitro. Using a western blot (WB) assay the humoral IgG responses, induced by
spp. larvae to the worm's E/S antigens in different infected hosts including mice, rabbits and human, were examined.
In a mouse model of toxocariasis, intraperitoneal injection of
larvae induces inflammatory leukocyte accumulation in the liver and the lungs but not in the brain, although a remarkable number of larvae were detected in this organ. Mice and rabbits responded differently to
spp. resulting in distinct heterogenous WB band patterns. Mice and rabbits both responded to a 33.1 kDa E/S constituent that turned out to be the most sensitive protein for serodiagnosis. Sera from human toxocariasis patients showed heterogenous WB band patterns similar to those observed in rabbits and all responded to the 33.1 kDa band.
33.1 kDa E/S protein can be considered as a critical common biomarker for toxocariasis immuno-diagnosis in both paratenic animals and human and its specificity requires further investigation.</abstract><cop>Iran</cop><pub>Tehran University of Medical Sciences</pub><pmid>28761463</pmid><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1735-7020 |
| ispartof | Iranian journal of parasitology, 2017, Vol.12 (1), p.69-82 |
| issn | 1735-7020 2008-238X |
| language | eng |
| recordid | cdi_doaj_primary_oai_doaj_org_article_1e7fd9e7343e4462a8dd71dcf2597702 |
| source | Publicly Available Content Database; PubMed Central |
| subjects | Animals Antigens Biomarkers Cytokines Eggs Excreted/secreted proteins IgG response Immunoglobulins Infections Molecular weight Nematodes Original Parasitology Paratenic hosts Pharmacy Proteins Toxocara |
| title | The 33.1 kDa Excretory/secretory Protein Produced by Toxocara canis Larvae Serves as a Potential Common Biomarker for Serodiagnosis of Toxocariasis in Paratenic Animals and Human |
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