Gramicidin, a Bactericidal Antibiotic, Is an Antiproliferative Agent for Ovarian Cancer Cells

: Gramicidin, a bactericidal antibiotic used in dermatology and ophthalmology, has recently garnered attention for its inhibitory actions against cancer cell growth. However, the effects of gramicidin on ovarian cancer cells and the underlying mechanisms are still poorly understood. We aimed to eluc...

Full description

Saved in:
Bibliographic Details
Published in:Medicina (Kaunas, Lithuania) Lithuania), 2023-11, Vol.59 (12), p.2059
Main Authors: Choi, Min Sung, Lee, Chae Yeon, Kim, Ji Hyeon, Lee, Yul Min, Lee, Sukmook, Kim, Hyun Jung, Heo, Kyun
Format: Article
Language:English
Subjects:
Angiogenesis
Anti-Bacterial Agents - pharmacology
Anti-Bacterial Agents - therapeutic use
Antibiotics
Apoptosis
Cancer
Cancer cells
Cancer therapies
Care and treatment
Cell cycle
Cell growth
Cell Line, Tumor
Cell Proliferation
Chemotherapy
Clinical trials
Computer software industry
DNA - pharmacology
Drug dosages
drug repositioning
Drug therapy
Ethanol
Ethylenediaminetetraacetic acid
Female
Flow cytometry
Gastric cancer
Gram-positive bacteria
gramicidin
Gramicidin - pharmacology
Gramicidin - therapeutic use
Humans
Kidney cancer
Mutation
Ovarian cancer
Ovarian Neoplasms - drug therapy
Ovarian Neoplasms - metabolism
Prevention
Proteins
Scientific equipment and supplies industry
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:: Gramicidin, a bactericidal antibiotic used in dermatology and ophthalmology, has recently garnered attention for its inhibitory actions against cancer cell growth. However, the effects of gramicidin on ovarian cancer cells and the underlying mechanisms are still poorly understood. We aimed to elucidate the anticancer efficacy of gramicidin against ovarian cancer cells. : The anticancer effect of gramicidin was investigated through an in vitro experiment. We analyzed cell proliferation, DNA fragmentation, cell cycle arrest and apoptosis in ovarian cancer cells using WST-1 assay, terminal deoxynucleotidyl transferase dUTP nick and labeling (TUNEL), DNA agarose gel electrophoresis, flow cytometry and western blot. : Gramicidin treatment induces dose- and time-dependent decreases in OVCAR8, SKOV3, and A2780 ovarian cancer cell proliferation. TUNEL assay and DNA agarose gel electrophoresis showed that gramicidin caused DNA fragmentation in ovarian cancer cells. Flow cytometry demonstrated that gramicidin induced cell cycle arrest. Furthermore, we confirmed via Western blot that gramicidin triggered apoptosis in ovarian cancer cells. : Our results strongly suggest that gramicidin exerts its inhibitory effect on cancer cell growth by triggering apoptosis. Conclusively, this study provides new insights into the previously unexplored anticancer properties of gramicidin against ovarian cancer cells.
ISSN:1648-9144
1010-660X
1648-9144
DOI:10.3390/medicina59122059