Expression Profiling and Functional Analysis of Candidate Odorant Receptors in Galeruca daurica

Galeruca daurica (Joannis) is an oligophagous pest in the grasslands of Inner Mongolia, China, which feed mainly on Allium spp. Odorant receptors (ORs) play an important role in the olfactory system in insects, and function together with olfactory co-receptor (ORco). In this study, 21 OR genes were...

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Bibliographic Details
Published in:Insects (Basel, Switzerland) Switzerland), 2022-06, Vol.13 (7), p.563
Main Authors: Zhang, Jing-Hang, Li, Ling, Li, Na, Li, Yan-Yan, Pang, Bao-Ping
Format: Article
Language:English
Subjects:
Adults
Allelochemicals
Antennae
Efficiency
electroantennogram
expression profile
Functional analysis
Galeruca daurica
Gene expression
Genes
Grasslands
Host plants
Insects
Odorant receptors
Olfaction
Olfactory system
Proteins
Receptors
RNA interference
RNA-mediated interference
Transcriptomes
Volatiles
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Summary:Galeruca daurica (Joannis) is an oligophagous pest in the grasslands of Inner Mongolia, China, which feed mainly on Allium spp. Odorant receptors (ORs) play an important role in the olfactory system in insects, and function together with olfactory co-receptor (ORco). In this study, 21 OR genes were identified from the transcriptome database of G. daurica adults, and named GdauOR1-20 and GdauORco. The expression profiles were examined by RT-qPCR and RNA interference (RNAi) and electroantennogram (EAG) experiments were conducted to further identify the olfactory functions of GdauOR4, GdauOR11, GdauOR15, and GdauORco. It was found that 15 GdauORs (OR1, OR3-6, OR8, OR11-13, OR15, OR17-20, and ORco) were mainly expressed in antennae, and the expression levels of GdauORs in adults were affected by age. When GdauOR4, GdauOR15, and GdauORco were silenced by RNAi, the electrophysiological responses to host plant volatiles were significantly decreased in G. daurica. This study lays a necessary foundation for clarifying the mechanism on finding host plants in G. daurica.
ISSN:2075-4450
2075-4450
DOI:10.3390/insects13070563